ArticleScientific reports2021
Detection and quantification of γ-H2AX using a dissociation enhanced lanthanide fluorescence immunoassay.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 41 citations in OpenAlex.
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- Application of an imaging flow cytometry γ-H2AX assay for biodosimetry using supervised machine learning.International journal of radiation biology · 2026Article
- Dynamic γ-H2AX response in blood lymphocytes for prediction of radiotherapy induced bladder toxicity in cervical cancer patients.The Indian journal of medical research · 2026Article
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- Assessment of DNA damage and expression variation of genes related to DNA damage in salt mine workers.Molecular genetics and genomics : MGG · 2025Article
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- Ruthenium(II) Complex with 1-Hydroxy-9,10-Anthraquinone Inhibits Cell Cycle Progression at G0/G1 and Induces Apoptosis in Melanoma Cells.Pharmaceuticals (Basel, Switzerland) · 2025Article
- Controlled labelling of tracer antibodies for time-resolved fluorescence-based immunoassays.Scientific reports · 2024Article
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Authors and funding
6 authors at 4 institutions in 1 country.
Funding
Abstract
Phosphorylation of the histone protein H2AX to form γ-H2AX foci directly represents DNA double-strand break formation. Traditional γ-H2AX detection involves counting individual foci within individual nuclei. The novelty of this work is the application of a time-resolved fluorescence assay using dissociation-enhanced lanthanide fluorescence immunoassay for quantitative measurements of γ-H2AX. For comparison, standard fluorescence detection was employed and analyzed either by bulk fluorescent measurements or by direct foci counting using BioTek Spot Count algorithm and Gen 5 software. Etoposide induced DNA damage in A549 carcinoma cells was compared across all test platforms. Time resolved fluorescence detection of europium as a chelated complex enabled quantitative measurement of γ-H2AX foci with nanomolar resolution. Comparative bulk fluorescent signals achieved only micromolar sensitivity. Lanthanide based immunodetection of γ-H2AX offers superior detection and a user-friendly workflow. These approaches have the potential to improve screening of compounds that either enhance DNA damage or protect against its deleterious effects.
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