Evidence map›Paper›PMID 33903116›Full record

ArticleBMJ open diabetes research & care2021

Exploring the GLP-1-GLP-1R axis in porcine pancreas and gastrointestinal tract in vivo by ex vivo autoradiography.

Elin Manell, Emmi Puuvuori, Anna Svensson, Irina Velikyan, Gry Hulsart-Billström, Patricia Hedenqvist, Jens Juul Holst, Marianne Jensen Waern, Olof Eriksson

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMJ open diabetes research & care, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.4field-weighted citation impact, top 36% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 2 countries.

Elin ManellDepartment of Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden elin.manell@slu.se.ORCID 0000-0002-4491-7609
Emmi PuuvuoriScience for Life Laboratory, Department of Medicinal Chemistry, Uppsala University, Uppsala, Sweden.
Anna SvenssonDepartment of Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Irina VelikyanScience for Life Laboratory, Department of Medicinal Chemistry, Uppsala University, Uppsala, Sweden.
Gry Hulsart-BillströmScience for Life Laboratory, Department of Medicinal Chemistry, Uppsala University, Uppsala, Sweden.
Patricia HedenqvistDepartment of Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Jens Juul HolstNNF Centre for Basic Metabolic Research and Department of Biomedical Sciences, University of Copenhagen, Copenhagen, Denmark.ORCID 0000-0001-6853-3805
Marianne Jensen WaernDepartment of Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Olof ErikssonScience for Life Laboratory, Department of Medicinal Chemistry, Uppsala University, Uppsala, Sweden.ORCID 0000-0002-2515-8790
Swedish University of Agricultural Sciences · SEUppsala University · SEUniversity of Copenhagen · DK

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionGlucagon-like peptide-1 (GLP-1) increases insulin secretion from pancreatic beta-cells and GLP-1 receptor (GLP-1R) agonists are widely used as treatment for type 2 diabetes mellitus. Studying occupancy of the GLP-1R in various tissues is challenging due to lack of quantitative, repeatable assessments of GLP-1R density. The present study aimed to describe the quantitative distribution of GLP-1Rs and occupancy by endogenous GLP-1 during oral glucose tolerance test (OGTT) in pigs, a species that is used in biomedical research to model humans. RESEARCH DESIGN AND

methodsGLP-1R distribution and occupancy were measured in pancreas and gastrointestinal tract by ex vivo autoradiography using the GLP-1R-specific radioligand

resultsHigh homogenous uptake of

conclusionWe identified areas with similarities as well as important differences regarding GLP-1R distribution and occupancy in pigs compared with humans. First, there was strong ligand binding in the exocrine pancreas in islets. Second, GLP-1 secretion during OGTT is minimal and GLP-1 might not be an important incretin in pigs under physiological conditions. These findings offer new insights on the relevance of porcine diabetes models.

Indexed as

Diabetes Mellitus, Type 2Glucagon-Like Peptide 1AnimalsAutoradiographyGastrointestinal TractPancreasSwineTissue DistributionGlucagon-Like Peptide 1gastrointestinal hormoneglucagon-like peptide 1glucose tolerance testincretinsreceptors

Identifiers

PMID33903116
PMCPMC8076945
OpenAlexW3158367088

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.