ArticleEnvironmental microbiome2021
The microbiome of the Black Sea water column analyzed by shotgun and genome centric metagenomics.
Article in Environmental microbiome, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
40 citing papers in PubMed.
- Denitrification Modularity and Its Environmental Controls in Transiently Versus Permanently Anoxic Marine Systems.Environmental microbiology · 2026Article
- Microbe Decoder uncovers functional traits of microbes in microbiome datasets.Nucleic acids research · 2026Article
- Improving metagenome binning by integrating intrinsic features and taxonomy.Nature biotechnology · 2026Article
- Spatial and temporal patterns of public transit aerobiomes.Microbiome · 2026Article
- Indicator bacteria in beaver ponds-research from Poland.Microbiology spectrum · 2026Article
- MagnetotacticISME communications · 2026Article
- Black Sea picocyanobacteria exploit ancestral traits for energy and chlorophyll production in dark anoxia.ISME communications · 2026Article
- Standardizing microbiome research: interlaboratory validation of SOPs for sample preparation and DNA extraction from food and environmental ecosystems.Environmental microbiome · 2025Article
- Dependence of Bacterial OTUs on Selected Features of Beaver Ponds.Ecology and evolution · 2025Article
- Methane filtration and metabolic cooperation of microbial communities in cold seep water columns from South China Sea.Communications biology · 2025Article
- Candidatus List No. 6.International journal of systematic and evolutionary microbiology · 2025Article
- Discovering Hidden Archaeal and Bacterial Lipid Producers in a Euxinic Marine System.Environmental microbiology · 2025Article
- Distinct microbial communities within and on seep carbonates support long-term anaerobic oxidation of methane and divergent pMMO diversity.The ISME journal · 2025Article
- Phylogenetic diversity and community structure ofFrontiers in microbiology · 2025Article
- Article
- Plasmid-Borne Biosynthetic Gene Clusters within a Permanently Stratified Marine Water Column.Microorganisms · 2024Article
- Improved sampling and DNA extraction procedures for microbiome analysis in food-processing environments.Nature protocols · 2024Article
- Novel Alphaproteobacteria transcribe genes for nitric oxide transformation at high levels in a marine oxygen-deficient zone.Applied and environmental microbiology · 2024Article
- Production of structurally diverse sphingolipids by anaerobic marine bacteria in the euxinic Black Sea water column.The ISME journal · 2024Article
- Metagenomic 18S rDNA reads revealed zonation of eukaryotic communities in the Yongle blue hole.Frontiers in microbiology · 2024Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors.
Funding
Abstract
backgroundThe Black Sea is the largest brackish water body in the world, although it is connected to the Mediterranean Sea and presents an upper water layer similar to some regions of the former, albeit with lower salinity and temperature. Despite its well-known hydrology and physicochemical features, this enormous water mass remains poorly studied at the microbial genomics level.
resultsWe have sampled its different water masses and analyzed the microbiome by shotgun and genome-resolved metagenomics, generating a large number of metagenome-assembled genomes (MAGs) from them. We found various similarities with previously described Black Sea metagenomic datasets, that show remarkable stability in its microbiome. Our datasets are also comparable to other marine anoxic water columns like the Cariaco Basin. The oxic zone resembles to standard marine (e.g. Mediterranean) photic zones, with Cyanobacteria (Synechococcus but a conspicuously absent Prochlorococcus), and photoheterotrophs domination (largely again with marine relatives). The chemocline presents very different characteristics from the oxic surface with many examples of chemolithotrophic metabolism (Thioglobus) and facultatively anaerobic microbes. The euxinic anaerobic zone presents, as expected, features in common with the bottom of meromictic lakes with a massive dominance of sulfate reduction as energy-generating metabolism, a few (but detectable) methanogenesis marker genes, and a large number of "dark matter" streamlined genomes of largely unpredictable ecology.
conclusionsThe Black Sea oxic zone presents many similarities to the global ocean while the redoxcline and euxinic water masses have similarities to other similar aquatic environments of marine (Cariaco Basin or other Black Sea regions) or freshwater (meromictic monimolimnion strata) origin. The MAG collection represents very well the different types of metabolisms expected in this kind of environment. We are adding critical information about this unique and important ecosystem and its microbiome.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.