ArticleInsect biochemistry and molecular biology2021
E93 confers steroid hormone responsiveness of digestive enzymes to promote blood meal digestion in the midgut of the mosquito Aedes aegypti.
Article in Insect biochemistry and molecular biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 54 citations in OpenAlex.
- RNA-seq Co-Expression Analysis Reveals a Midgut-Associated Digestive Gene Module inBiotech (Basel (Switzerland)) · 2026Article
- Present and Future of Mosquito-Borne Disease Control in Europe with a Specific Focus on the Mediterranean.Insects · 2026Review
- Temporal changes in amino acid profiles coordinate mosquito physiology and egg maturation in Aedes aegypti.BMC biology · 2025Article
- Genomic and insulin-mediated control of metabolic homeostasis by the mosquito ecdysone-induced gene E93.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Insulin-like peptides activate egg formation in the Asian malaria mosquito Anopheles stephensi.Parasites & vectors · 2025Article
- Single-cell transcriptional landscapes of Aedes aegypti midgut and fat body after a bloodmeal.Cell genomics · 2025Article
- Improving the Capstone Biochemistry Lab and Evolution to a Course-Based Undergraduate Research Experience: Lessons Learned from the COVID-19 Online Modality.Journal of college science teaching · 2025Article
- Biochemical and physiological characterization of Aedes aegypti midgut chymotrypsin.Scientific reports · 2025Article
- 20-Hydroxyecdysone Regulates the Expression of 30 Genes Specifically Expressed in Larval Digestive Tube of the Silkworm,Insects · 2025Article
- Comprehensive proteolytic profiling of Aedes aegypti mosquito midgut extracts: Unraveling the blood meal protein digestion system.PLoS neglected tropical diseases · 2025Article
- Transcription factor E93 regulates vitellogenesis via the vitelline membrane protein 26Ab gene in Chilo Suppressalis.Molecular biology reports · 2024Article
- Reciprocal interactions between neuropeptide F and RYamide regulate host attraction in the mosquitoProceedings of the National Academy of Sciences of the United States of America · 2024Article
- The transcriptional response in mosquitoes distinguishes between fungi and bacteria but not Gram types.BMC genomics · 2024Article
- Insulin-like peptides and ovary ecdysteroidogenic hormone differentially stimulate physiological processes regulating egg formation in the mosquito Aedes aegypti.Insect biochemistry and molecular biology · 2023Article
- A transcriptomic atlas ofeLife · 2022Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
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Abstract
Anautogenous female mosquitoes obtain the nutrients needed for egg development from vertebrate blood, and consequently they transmit numerous pathogens of devastating human diseases. Digestion of blood proteins into amino acids that are used for energy production, egg maturation and replenishment of maternal reserves is an essential part of the female mosquito reproductive cycle. However, the regulatory mechanisms underlying this process remain largely unknown. Here, we report that the transcription factor E93 is a critical factor promoting blood meal digestion in adult females of the major arboviral vector Aedes aegypti in response to the steroid hormone 20-hydroxyecdysone (20E). E93 was upregulated in the female mosquito midgut after a blood meal, and RNA interference (RNAi)-mediated knockdown of E93 inhibited midgut blood digestion. E93 RNAi depletion repressed late trypsin (LT), serine protease I (SPI), SPVI and SPVII, and activated early trypsin (ET) expression in the female mosquito midgut after a blood meal. Injection of 20E activated E93, LT, SPI, SPVI and SPVII, and repressed ET expression, whereas RNAi knockdown of the ecdysone receptor (EcR) repressed E93, LT, SPI, SPVI and SPVII, and activated ET expression in the midgut. Furthermore, E93 depletion resulted in a complete loss of 20E responsiveness of LT, SPVI and SPVII. Our findings reveal important mechanisms regulating blood meal digestion in disease-transmitting mosquitoes.
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