Evidence map›Paper›PMID 33901254›Full record

ArticlePloS one2021

Small extracellular vesicle-encapsulated miR-181b-5p, miR-222-3p and let-7a-5p: Next generation plasma biopsy-based diagnostic biomarkers for inflammatory breast cancer.

Sarah Hamdy Ahmed, Nancy A Espinoza-Sánchez, Ahmed El-Damen, Sarah Atef Fahim, Mohamed A Badawy, Burkhard Greve, Mohamed El-Shinawi, Martin Götte, Sherif Abdelaziz Ibrahim

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
29citing papers in PubMed, 2 pooled it
2.8field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

29 citing papers in PubMed, 2 syntheses or guidelines pooled it, 41 citations in OpenAlex.

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  20. MiR-222-3p Aggravates the Inflammatory Response by Targeting SOCS1 to Activate STAT3 Signaling in Ulcerative Colitis.The Turkish journal of gastroenterology : the official journal of Turkish Society of Gastroenterology · 2022
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 2 countries.

Sarah Hamdy AhmedBiotechnology/Biomolecular Chemistry Program, Chemistry Department, Faculty of Science, Cairo University, Giza, Egypt.ORCID 0000-0002-2560-4172
Nancy A Espinoza-SánchezDepartment of Gynecology and Obstetrics, Münster University Hospital, Münster, Germany.
Ahmed El-DamenDepartment of Zoology, Faculty of Science, Cairo University, Giza, Egypt.ORCID 0000-0003-4830-425X
Sarah Atef FahimBiochemistry Program, Chemistry Department, Faculty of Science, Cairo University, Giza, Egypt.
Mohamed A BadawyChemistry Department, Faculty of Science, Cairo University, Giza, Egypt.
Burkhard GreveDepartment of Radiotherapy-Radiooncology, University Hospital Münster, Münster, Germany.
Mohamed El-ShinawiGalala University, Suez, Egypt.
Martin GötteDepartment of Gynecology and Obstetrics, Münster University Hospital, Münster, Germany.ORCID 0000-0003-2360-2496
Sherif Abdelaziz IbrahimDepartment of Zoology, Faculty of Science, Cairo University, Giza, Egypt.ORCID 0000-0001-6403-7345
Cairo University · EGUniversity Hospital Münster · DEAin Shams University · EG

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Inflammatory breast cancer (IBC) is a rare, but aggressive entity of breast carcinoma with rapid dermal lymphatic invasion in young females. It is either poorly or misdiagnosed as mastitis because of the absence of a distinct lump. Small extracellular vesicles (sEVs) circulating in liquid biopsies are a novel class of minimally invasive diagnostic alternative to invasive tissue biopsies. They modulate cancer progression via shuttling their encapsulated cargo including microRNAs (miRNAs) into recipient cells to either trigger signaling or induce malignant transformation of targeted cells. Plasma sEVs < 200 nm were isolated using a modified cost-effective polyethylene glycol (PEG)-based precipitation method and compared to standard methods, namely ultracentrifugation and a commercial kit, where the successful isolation was verified by different approaches. We evaluated the expression levels of selected sEV-derived miR-181b-5p, miR-222-3p and let-7a-5p using quantitative real PCR (qPCR). Relative to non-IBC, our qPCR data showed that sEV-derived miR-181b-5p and miR-222-3p were significantly upregulated, whereas let-7a-5p was downregulated in IBC patients. Interestingly, receiver operating characteristic (ROC) curves analysis revealed that diagnostic accuracy of let-7a-5p alone was the highest for IBC with an area under curve (AUC) value of 0.9188, and when combined with miR-222-3p the AUC was improved to 0.973. Further, 38 hub genes were identified using bioinformatics analysis. Together, circulating sEV-derived miR-181b-5p, miR-222-3p and let-7a-5p serve as promising non-invasive diagnostic biomarkers for IBC.

Indexed as

AdultAgedArea Under CurveBiomarkers, TumorExtracellular VesiclesFemaleHumansInflammatory Breast NeoplasmsLiquid BiopsyMicroRNAsMiddle AgedProtein Interaction MapsReal-Time Polymerase Chain ReactionROC CurveSensitivity and SpecificityTranscriptomeBiomarkers, TumorMicroRNAsMIR222, humanMIrn181 microRNA, humanmirnlet7 microRNA, human

Identifiers

PMID33901254
PMCPMC8075236
OpenAlexW3158700720

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.