ArticleEmerging infectious diseases2021
Monitoring SARS-CoV-2 Circulation and Diversity through Community Wastewater Sequencing, the Netherlands and Belgium.
Article in Emerging infectious diseases, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 137 papers, 1 of them a synthesis that pooled it.
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Who cites it
137 citing papers in PubMed, 1 synthesis or guideline pooled it.
- A Systematic Review of Methodological Approaches to SARS-CoV-2 Wastewater Surveillance.Viruses · 2026Pooled it
- Long-Term Wastewater Genomic Surveillance Reveals SARS-CoV-2 Variant Dynamics in Italy from 2021 to 2025.Food and environmental virology · 2026Article
- A comprehensive database for biological data derived from sewage in five European cities.Database : the journal of biological databases and curation · 2026Article
- SARS-CoV-2 wastewater genomic surveillance: approaches, challenges, and opportunities.Genome biology · 2026Review
- Unsupervised detection and fitness estimation of emerging SARS-CoV-2 variants: Application to wastewater samples (ANRS0160).PLoS computational biology · 2025Article
- Unveiling the global urban virome through wastewater metagenomics.Nature communications · 2025Article
- A case study using sewage metagenomic data for assessment of text-to-SQL capabilities in large language models.Scientific reports · 2025Article
- Comprehensive wastewater genomic surveillance in the Netherlands: insights into country-wide SARS-CoV-2 lineage dynamics and implications for future surveillance.BMC public health · 2025Article
- Human mobility and sewage data correlate with COVID-19 epidemic evolution in a 3-year surveillance of the metropolitan area of Bologna.BMC infectious diseases · 2025Article
- Optimization of the Primary Sludge Processing Method for Wastewater Genomic Surveillance of SARS-CoV‑2.ACS ES&T water · 2025Article
- Identification of circulating human papillomavirus types through high-throughput sequencing of Canadian municipal and institutional wastewater samples.Applied and environmental microbiology · 2025Article
- Early detection of emerging SARS-CoV-2 Variants from wastewater through genome sequencing and machine learning.Nature communications · 2025Article
- Diagnostic performance of allele-specific RT-qPCR and genomic sequencing in wastewater-based surveillance of SARS-CoV-2.Eco-Environment & Health · 2025Article
- Hydrological and physicochemical parameters associated with SARS-CoV-2 and pepper mild mottle virus wastewater concentrations for a large-combined sewer system.Journal of water and health · 2025Article
- VILOCA: sequencing quality-aware viral haplotype reconstruction and mutation calling for short-read and long-read data.NAR genomics and bioinformatics · 2024Article
- Tracking SARS-CoV-2 variants in wastewater in San Pedro de la Paz, Chile.Journal of water and health · 2024Article
- Agile, on-demand wastewater surveillance of virus infections to support pandemic and outbreak response in Rotterdam-Rijnmond, the Netherlands, 2020 to 2022.Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2024Article
- A Narrative Review of High Throughput Wastewater Sample Processing for Infectious Disease Surveillance: Challenges, Progress, and Future Opportunities.International journal of environmental research and public health · 2024Review
- Tracking epidemic viruses in wastewaters.Microbial biotechnology · 2024Review
- The first detection of SARS-CoV-2 RNA in the wastewater of Bucharest, Romania.Scientific reports · 2024Article
77 more citing papers are in PubMed but not listed here.
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Authors and funding
13 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has rapidly become a major global health problem, and public health surveillance is crucial to monitor and prevent virus spread. Wastewater-based epidemiology has been proposed as an addition to disease-based surveillance because virus is shed in the feces of ≈40% of infected persons. We used next-generation sequencing of sewage samples to evaluate the diversity of SARS-CoV-2 at the community level in the Netherlands and Belgium. Phylogenetic analysis revealed the presence of the most prevalent clades (19A, 20A, and 20B) and clustering of sewage samples with clinical samples from the same region. We distinguished multiple clades within a single sewage sample by using low-frequency variant analysis. In addition, several novel mutations in the SARS-CoV-2 genome were detected. Our results illustrate how wastewater can be used to investigate the diversity of SARS-CoV-2 viruses circulating in a community and identify new outbreaks.
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