Evidence map›Paper›PMID 33899435›Full record

ArticleSheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi2021

[Rapid screening of single guide RNA targeting pig genome and the harvesting of monoclonal cells by microarray seal].

Mengyu Gao, Xinglong Zhu, Shisheng Wang, Bingqi Zhang, Yunlin Zhang, Yuting He, Yanyan Zhou, Shun Li, Guang Yang, Guangneng Liao and 2 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 97% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 2 institutions in 1 country.

Mengyu GaoDepartment of Pathology, West China Hospital, Sichuan University, Chengdu 610041, P.R.China;Institute of Clinical Pathology, West China Hospital, Sichuan University, Chengdu 610041, P.R.China.
Xinglong ZhuInstitute of Clinical Pathology, West China Hospital, Sichuan University, Chengdu 610041, P.R.China;Laboratory of Pathology, Key Laboratory of Transplant Engineering and Immunology, NHFPC; West China Hospital, Sichuan University, Chengdu 610041, P.R.China.
Shisheng WangWest China - Washington Mitochondria and Metabolism Research Center, West China Hospital, Sichuan University, Chengdu 610041, P.R.China.
Bingqi ZhangInstitute of Clinical Pathology, West China Hospital, Sichuan University, Chengdu 610041, P.R.China;Laboratory of Pathology, Key Laboratory of Transplant Engineering and Immunology, NHFPC; West China Hospital, Sichuan University, Chengdu 610041, P.R.China.
Yunlin ZhangInstitute of Clinical Pathology, West China Hospital, Sichuan University, Chengdu 610041, P.R.China;Laboratory of Pathology, Key Laboratory of Transplant Engineering and Immunology, NHFPC; West China Hospital, Sichuan University, Chengdu 610041, P.R.China.
Yuting HeInstitute of Clinical Pathology, West China Hospital, Sichuan University, Chengdu 610041, P.R.China;Laboratory of Pathology, Key Laboratory of Transplant Engineering and Immunology, NHFPC; West China Hospital, Sichuan University, Chengdu 610041, P.R.China.
Yanyan ZhouInstitute of Clinical Pathology, West China Hospital, Sichuan University, Chengdu 610041, P.R.China.
Shun LiDepartment of Biophysics, School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 611731, P.R.China.
Guang YangExperimental Animal Center, West China Hospital, Sichuan University, Chengdu 610041, P.R.China.
Guangneng LiaoExperimental Animal Center, West China Hospital, Sichuan University, Chengdu 610041, P.R.China.
Ji BaoInstitute of Clinical Pathology, West China Hospital, Sichuan University, Chengdu 610041, P.R.China;Laboratory of Pathology, Key Laboratory of Transplant Engineering and Immunology, NHFPC; West China Hospital, Sichuan University, Chengdu 610041, P.R.China.
Hong BuDepartment of Pathology, West China Hospital, Sichuan University, Chengdu 610041, P.R.China;Institute of Clinical Pathology, West China Hospital, Sichuan University, Chengdu 610041, P.R.China.
Sichuan University · CNUniversity of Electronic Science and Technology of China · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The emergence of regular short repetitive palindromic sequence clusters (CRISPR) and CRISPR- associated proteins 9 (Cas9) gene editing technology has greatly promoted the wide application of genetically modified pigs. Efficient single guide RNA (sgRNA) is the key to the success of gene editing using CRISPR/Cas9 technology. For large animals with a long reproductive cycle, such as pigs, it is necessary to screen out efficient sgRNA

Indexed as

CRISPR-Cas SystemsRNA, Guide, CRISPR-Cas SystemsAnimalsClustered Regularly Interspaced Short Palindromic RepeatsGene EditingSwineRNA, Guide, CRISPR-Cas Systemsfumarylacetoacetate hydrolase gene knockoutgene editing for large animalspatterned microarrayrapid screening of sgRNAregular short repetitive Palindromic sequence clusters and CRISPR- associated proteins 9

Identifiers

PMID33899435
PMCPMC10307559
OpenAlexW3159336589

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.