ArticleBiology of reproduction2021
Importance of WNT-dependent signaling for derivation and maintenance of primed pluripotent bovine embryonic stem cells†.
Article in Biology of reproduction, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 28 citations in OpenAlex.
- A feeder-free culture system supports long-term expansion and germline competence of bovine formative embryonic stem cells†.Biology of reproduction · 2026Article
- Effect of Small Molecules on Blastocyst Development and Outgrowth Establishment of Bovine Haploid Parthenogenetic Embryos.Animals : an open access journal from MDPI · 2026Article
- Genomic ancestry and adaptive signatures in the indigenous Hetian cattle from Xinjiang Province of China revealed by whole-genome sequencing.BMC genomics · 2025Article
- MicroRNA (miRNA) in Plasma Small Extracellular Vesicles (sEV) as Potential Early Indicators of Dairy Cow Subfertility.Journal of extracellular biology · 2025Article
- Tracing and Capturing the Epiblast Pluripotency of Sheep Preimplantation Embryos.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Advancements in Stem Cell Applications for Livestock Research: A Review.Veterinary sciences · 2025Review
- The Role of BDNF, YBX1, CENPF, ZSCAN4, TEAD4, GLIS1 and USF1 in the Activation of the Embryonic Genome in Bovine Embryos.International journal of molecular sciences · 2023Review
- Presence of KREMEN receptors for DKK1 in the preimplantation bovine embryo.Reproduction & fertility · 2023Article
- Inhibition of Wnt activity improves peri-implantation development of somatic cell nuclear transfer embryos.National science review · 2023Article
- Lab partners: oocytes, embryos and company. A personal view on aspects of oocyte maturation and the development of monozygotic twins.Animal reproduction · 2023Article
- Actions of WNT family member 5A to regulate characteristics of development of the bovine preimplantation embryo†.Biology of reproduction · 2022Article
- Pluripotent Core in Bovine Embryos: A Review.Animals : an open access journal from MDPI · 2022Review
- Regulation of NANOG and SOX2 expression by activin A and a canonical WNT agonist in bovine embryonic stem cells and blastocysts.Biology open · 2021Article
- Atlas of receptor genes expressed by the bovine morula and corresponding ligand-related genes expressed by uterine endometrium.Molecular reproduction and development · 2021Article
- Cytokines That Serve as Embryokines in Cattle.Animals : an open access journal from MDPI · 2021Review
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
Abstract
The WNT signaling system plays an important but paradoxical role in the regulation of pluripotency. In the cow, IWR-1, which inhibits canonical WNT activation and has WNT-independent actions, promotes the derivation of primed pluripotent embryonic stem cells from the blastocyst. Here, we describe a series of experiments to determine whether derivation of embryonic stem cells could be generated by replacing IWR-1 with other inhibitors of WNT signaling. Results confirm the importance of inhibition of canonical WNT signaling for the establishment of pluripotent embryonic stem cells in cattle and indicate that the actions of IWR-1 can be mimicked by the WNT secretion inhibitor IWP2 but not by the tankyrase inhibitor XAV939 or WNT inhibitory protein dickkopf 1. The role of Janus kinase-mediated signaling pathways for the maintenance of pluripotency of embryonic stem cells was also evaluated. Maintenance of pluripotency of embryonic stem cells lines was blocked by a broad inhibitor of Janus kinase, even though the cells did not express phosphorylated signal transducer and activator of transcription 3 (pSTAT3). Further studies with blastocysts indicated that IWR-1 blocks the activation of pSTAT3. A likely explanation is that IWR-1 blocks differentiation of embryonic stem cells into a pSTAT3+ lineage. In conclusion, results presented here indicate the importance of inhibition of WNT signaling for the derivation of pluripotent bovine embryonic stem cells, the role of Janus kinase signaling for maintenance of pluripotency, and the participation of IWR-1 in the inhibition of activation of STAT3.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.