Evidence map›Paper›PMID 33898972›Full record

ArticlePlant communications2021

Highly efficient and genotype-independent barley gene editing based on anther culture.

Yong Han, Sue Broughton, Li Liu, Xiao-Qi Zhang, Jianbin Zeng, Xiaoyan He, Chengdao Li

Abstract read
In one paragraph

Article in Plant communications, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.

0numbers the graph read from it
0cells of the map it votes in
30citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

30 citing papers in PubMed.

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  8. MicroRNA164d suppresses the HvNAC92-HvHKT1;5 module to enhance salinity tolerance in barley.Proceedings of the National Academy of Sciences of the United States of America · 2025
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  19. Evaluation of CRISPR/Cas9 Constructs in Wheat Cell Suspension Cultures.International journal of molecular sciences · 2023
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Yong HanWestern Barley Genetics Alliance, College of Science, Health, Engineering and Education, Murdoch University, Murdoch, WA 6150, Australia.
Sue BroughtonDepartment of Primary Industries and Regional Development, 3 Baron-Hay Court, South Perth, WA 6151, Australia.
Li LiuDepartment of Primary Industries and Regional Development, 3 Baron-Hay Court, South Perth, WA 6151, Australia.
Xiao-Qi ZhangWestern Barley Genetics Alliance, College of Science, Health, Engineering and Education, Murdoch University, Murdoch, WA 6150, Australia.
Jianbin ZengCollege of Agronomy, Qingdao Agricultural University, Qingdao, Shandong 266109, China.
Xiaoyan HeCollege of Agronomy, Qingdao Agricultural University, Qingdao, Shandong 266109, China.
Chengdao LiWestern Barley Genetics Alliance, College of Science, Health, Engineering and Education, Murdoch University, Murdoch, WA 6150, Australia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Recalcitrance to tissue culture and genetic transformation is the major bottleneck for gene manipulation in crops. In barley, immature embryos of Golden Promise have typically been used as explants for transformation. However, the genotype dependence of this approach limits the genetic modification of commercial varieties. Here, we developed an anther culture-based system that permits the effective creation of transgenic and gene-edited plants from commercial barley varieties. The protocol was tested in Golden Promise and four Australian varieties, which differed in phenology, callus induction, and green plant regeneration responses.

Indexed as

GenotypeCells, CulturedFlowersGene EditingHordeumPlant CellsAgrobacteriumCRISPRgenetic transformationHordeum vulgareoff-targettargeted mutation

Identifiers

PMID33898972
PMCPMC8060703

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.