Evidence map›Paper›PMID 33898871›Full record

ArticleBioactive materials2021

Study the lipidoid nanoparticle mediated genome editing protein delivery using 3D intestinal tissue model.

Tao Yang, Haobo Han, Ying Chen, Liu Yang, Rachael Parker, Yamin Li, David L Kaplan, Qiaobing Xu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bioactive materials, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.4field-weighted citation impact, top 41% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Targeted nanodelivery systems for personalized cancer therapy.Reports of practical oncology and radiotherapy : journal of Greatpoland Cancer Center in Poznan and Polish Society of Radiation Oncology · 2024
    Review
  5. Review
  6. Challenges in delivering therapeutic peptides and proteins: A silk-based solution.Journal of controlled release : official journal of the Controlled Release Society · 2022
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 2 countries.

Tao YangDepartment of Biomedical Engineering, Tufts University, Medford, MA, USA.
Haobo HanDepartment of Biomedical Engineering, Tufts University, Medford, MA, USA.
Ying ChenDepartment of Biomedical Engineering, Tufts University, Medford, MA, USA.
Liu YangDepartment of Biomedical Engineering, Tufts University, Medford, MA, USA.
Rachael ParkerDepartment of Biomedical Engineering, Tufts University, Medford, MA, USA.
Yamin LiDepartment of Biomedical Engineering, Tufts University, Medford, MA, USA.
David L KaplanDepartment of Biomedical Engineering, Tufts University, Medford, MA, USA.
Qiaobing XuDepartment of Biomedical Engineering, Tufts University, Medford, MA, USA.
Tufts University · US

Funding

Interaction of enteropathogenic Yersinia and Vibrio cholera with engineered human intestinal tissueU19AI131126 · NIAID · TUFTS UNIVERSITY BOSTON · PI KAPLAN, DAVID L. · 2017 to 2021
$8.1M
ROS-responsive Chemical Modification of Protein and Its DeliveryR01EB027170 · NIBIB · TUFTS UNIVERSITY MEDFORD · PI XU, QIAOBING · 2018 to 2022
$1.4M
NIAID NIH HHS U19 AI131126NIBIB NIH HHS R01 EB027170
6 · The paper itself

Abstract

Lipid nanoparticles are promising carriers for oral drug delivery. For bioactive cargos with intracellular targets, e.g. gene-editing proteins, it is essential for the cargo and carrier to remain complexed after crossing the epithelial layer of intestine in order for the delivery system to transport the cargos inside targeted cells. However, limited studies have been conducted to verify the integrity of cargo/carrier nanocomplexes and their capability in facilitating cargo delivery intracellularly after the nanocomplex crossing the epithelial barrier. Herein, we used a traditional 2D transwell system and a recently developed 3D tissue engineered intestine model and demonstrated the synthetic lipid nanoparticle (carrier) and protein (cargo) nanocomplexes are able to cross the epithelial layer and deliver the protein cargo inside the underneath cells. We found that the EC16-63 LNP efficiently encapsulated the GFP-Cre recombinase, penetrated the intestinal monolayer cells in both the 2D cell culture and 3D tissue models through temporarily interrupting the tight junctions between epithelial layer. After transporting across the intestinal epithelia, the EC16-63 and GFP-Cre recombinase nanocomplexes can enter the underneath cells to induce gene recombination. These results suggest that the in vitro 3D intestinal tissue model is useful for identifying effective lipid nanoparticles for potential oral drug delivery.

Indexed as

3D tissue modelGenome engineeringLipidoid nanoparticleOral drug deliveryProtein delivery

Identifiers

PMID33898871
PMCPMC8056182
OpenAlexW3159025024

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.