ArticleComputational and structural biotechnology journal2021
A strategy for complete telomere-to-telomere assembly of ciliate macronuclear genome using ultra-high coverage Nanopore data.
Article in Computational and structural biotechnology journal, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 22 citations in OpenAlex.
- Transposon targeting non-coding RNA transcription targets G/C-rich tracts and is facilitated by an intrinsically disordered protein in Tetrahymena.Science China. Life sciences · 2026Article
- A mechanism for telomere-specific telomere length regulation.Nucleic acids research · 2026Article
- Linear covariation between germline and somatic mutation rates across ciliates and mammals.Current biology : CB · 2025Article
- A mechanism for telomere-specific telomere length regulation.bioRxiv : the preprint server for biology · 2025Article
- Importin-9 and a TPR domain protein MpH drive periodic patterning of ciliary arrays in Tetrahymena.The Journal of cell biology · 2025Article
- A specialized TFIIB is required for transcription of transposon-targeting noncoding RNAs.Nucleic acids research · 2025Article
- Chromosome-level genome assembly of Cryptosporidium parvum by long-read sequencing of ten oocysts.Scientific data · 2024Article
- Genome assembly of a symbiotic balantidia (Balantidium ctenopharyngodoni) in fish hindgut.Scientific data · 2024Article
- From germline genome to highly fragmented somatic genome: genome-wide DNA rearrangement during the sexual process in ciliated protists.Marine life science & technology · 2024Article
- Programmed chromosome fragmentation in ciliated protozoa: multiple means to chromosome ends.Microbiology and molecular biology reviews : MMBR · 2023Review
- Group-specific functional patterns of mitochondrion-related organelles shed light on their multiple transitions from mitochondria in ciliated protists.Marine life science & technology · 2022Article
- A practical reference for studying meiosis in the model ciliateMarine life science & technology · 2022Review
- Zfp1, a Cys2His2 zinc finger protein is required for meiosis initiation inCell cycle (Georgetown, Tex.) · 2022Article
- SIGAR: Inferring Features of Genome Architecture and DNA Rearrangements by Split-Read Mapping.Genome biology and evolution · 2020Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ciliates contain two kinds of nuclei: the germline micronucleus (MIC) and the somatic macronucleus (MAC) in a single cell. The MAC usually have fragmented chromosomes. These fragmented chromosomes, capped with telomeres at both ends, could be gene size to several megabases in length among different ciliate species. So far, no telomere-to-telomere assembly of entire MAC genome in ciliate species has been finished. Development of the third generation sequencing technologies allows to generate sequencing reads up to megabases in length that could possibly span an entire MAC chromosome. Taking advantage of the ultra-long Nanopore reads, we established a simple strategy for the complete assembly of ciliate MAC genomes. Using this strategy, we assembled the complete MAC genomes of two ciliate species
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