ArticleEvolutionary applications2021
Design and analysis of CRISPR-based underdominance toxin-antidote gene drives.
Article in Evolutionary applications, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 58 citations in OpenAlex.
- Long-term stability and performance of Cas9/guide RNA-based gene drives in anopheline mosquitoes.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Advances in CRISPR gene drives for mosquito population control.Current opinion in microbiology · 2026Review
- Seed dormancy shapes gene drive dynamics in plants.Nature plants · 2026Article
- Harnessing the highly adaptable barnase-barstar system for genetic biocontrol of Aedes aegypti.Communications biology · 2025Article
- A Comparative Assessment of Self-limiting Genetic Control Strategies for Population Suppression.Molecular biology and evolution · 2025Article
- Improving the suppressive power of homing gene drive by co-targeting a distant-site female fertility gene.Nature communications · 2024Article
- Cleave and Rescue gamete killers create conditions for gene drive in plants.Nature plants · 2024Article
- Performance characteristics allow for confinement of a CRISPR toxin-antidote gene drive for population suppression in a reaction-diffusion model.Proceedings. Biological sciences · 2024Article
- Germline Cas9 promoters with improved performance for homing gene drive.Nature communications · 2024Article
- Modelling daisy quorum drive: A short-term bridge across engineered fitness valleys.PLoS genetics · 2024Article
- Cleave and Rescue gamete killers create conditions for gene drive in plants.bioRxiv : the preprint server for biology · 2024Article
- A framework for identifying fertility gene targets for mammalian pest control.Molecular ecology resources · 2024Article
- CRISPR-Based Therapies: Revolutionizing Drug Development and Precision Medicine.Current gene therapy · 2024Review
- Manipulating the Destiny of Wild Populations Using CRISPR.Annual review of genetics · 2023Review
- Harnessing Wolbachia cytoplasmic incompatibility alleles for confined gene drive: A modeling study.PLoS genetics · 2023Article
- Assessment of distant-site rescue elements for CRISPR toxin-antidote gene drives.Frontiers in bioengineering and biotechnology · 2023Article
- Fitness effects of CRISPR endonucleases ineLife · 2022Article
- Slow and steady wins the race: Spatial and stochastic processes and the failure of suppression gene drives.Molecular ecology · 2022Article
- A homing suppression gene drive with multiplexed gRNAs maintains high drive conversion efficiency and avoids functional resistance alleles.G3 (Bethesda, Md.) · 2022Article
- Experimental demonstration of tethered gene drive systems for confined population modification or suppression.BMC biology · 2022Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
CRISPR gene drive systems offer a mechanism for transmitting a desirable transgene throughout a population for purposes ranging from vector-borne disease control to invasive species suppression. In this simulation study, we assess the performance of several CRISPR-based underdominance gene drive constructs employing toxin-antidote (TA) principles. These drives disrupt the wild-type version of an essential gene using a CRISPR nuclease (the toxin) while simultaneously carrying a recoded version of the gene (the antidote). Drives of this nature allow for releases that could be potentially confined to a desired geographic location. This is because such drives have a nonzero-invasion threshold frequency required for the drive to spread through the population. We model drives which target essential genes that are either haplosufficient or haplolethal, using nuclease promoters with expression restricted to the germline, promoters that additionally result in cleavage activity in the early embryo from maternal deposition, and promoters that have ubiquitous somatic expression. We also study several possible drive architectures, considering both "same-site" and "distant-site" systems, as well as several reciprocally targeting drives. Together, these drive variants provide a wide range of invasion threshold frequencies and options for both population modification and suppression. Our results suggest that CRISPR TA underdominance drive systems could allow for the design of flexible and potentially confinable gene drive strategies.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.