ReviewFrontiers in neuroscience2021
Current Methods to Investigate Nociception and Pain in Zebrafish.
Review in Frontiers in neuroscience, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 44 citations in OpenAlex.
- Article
- Effects of analgesia on the response to a noxious stimulus in Norway lobsters (Nephrops norvegicus).Scientific reports · 2026Article
- Tricaine methanesulfonate and eugenol during semen collection do not affect fertilization and hatching rates onAnimal reproduction · 2026Article
- Antinociceptive and Anti-inflammatory Effect of Nicandrin B Isolated From Datura ferox in Zebrafish.Chemistry & biodiversity · 2025Article
- Advancing Zebrafish (Veterinary sciences · 2025Review
- Characterization of a chemically induced osteoarthritis model in zebrafish.Scientific reports · 2025Article
- Systematized Serendipity: Fishing Expeditions for Anesthetic Drugs and Targets.Anesthesiology · 2024Review
- Pain management in zebrafish : Report from a FELASA Working Group.Laboratory animals · 2024Review
- Antinociceptive Analysis of Natural Monoterpenes Eugenol, Menthol, Carvacrol and Thymol in a Zebrafish Larval Model.Pharmaceuticals (Basel, Switzerland) · 2024Article
- Assessment of the effect of tricaine (MS-222)-induced anesthesia on brain-wide neuronal activity of zebrafish (Frontiers in neuroscience · 2024Article
- Review
- How Instructions, Learning, and Expectations Shape Pain and Neurobiological Responses.Annual review of neuroscience · 2023Review
- Reversal of Neuralgia Effect of Beta Carotene in Streptozotocin-Associated Diabetic Neuropathic Pain in Female Zebrafish via Matrix Metalloprotease-13 Inhibition.Pharmaceuticals (Basel, Switzerland) · 2023Article
- A scalable assay for chemical preference of small freshwater fish.Frontiers in behavioral neuroscience · 2022Article
- Zebrafish Embryos and Larvae as Alternative Animal Models for Toxicity Testing.International journal of molecular sciences · 2021Review
- Nociceptive pain and anxiety in equines: Physiological and behavioral alterations.Veterinary world · 2021Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pain is an unpleasant, negative emotion and its debilitating effects are complex to manage. Mammalian models have long dominated research on nociception and pain, but there is increasing evidence for comparable processes in fish. The need to improve existing pain models for drug research and the obligation for 3R refinement of fish procedures facilitated the development of numerous new assays of nociception and pain in fish. The zebrafish is already a well-established animal model in many other research areas like toxicity testing, as model for diseases or regeneration and has great potential in pain research, too. Methods of electrophysiology, molecular biology, analysis of reflexive or non-reflexive behavior and fluorescent imaging are routinely applied but it is the combination of these tools what makes the zebrafish model so powerful. Simultaneously, observing complex behavior in free-swimming larvae, as well as their neuronal activity at the cellular level, opens new avenues for pain research. This review aims to supply a toolbox for researchers by summarizing current methods to study nociception and pain in zebrafish. We identify treatments with the best algogenic potential, be it chemical, thermal or electric stimuli and discuss options of analgesia to counter effects of nociception and pain by opioids, non-steroidal anti-inflammatory drugs (NSAIDs) or local anesthetics. In addition, we critically evaluate these practices, identify gaps of knowledge and outline potential future developments.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.