ArticleThe Journal of veterinary medical science2021
Resveratrol supplementation into extender protects against cryodamage in dog post-thaw sperm.
Article in The Journal of veterinary medical science, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 20 citations in OpenAlex.
- Nicotinic and Folic Acid Supplementation Improves Cryopreserved Ram Sperm Quality, Potentially Through Modulation ofInternational journal of molecular sciences · 2026Article
- Current Advances in Cetacean Semen Cryopreservation and Their Application to Yangtze Finless Porpoise Conservation.Animals : an open access journal from MDPI · 2026Review
- Protective Effect of Intracellular Compounds of Lactobacillus plantarum on Goat Sperm Quality During Cryopreservation.Veterinary medicine and science · 2026Article
- Astragalus polysaccharide improves canine sperm quality after chilled storage via promoting AMPK phosphorylation.BMC veterinary research · 2026Article
- Effects of resveratrol supplementation to equine sperm during semen cryopreservation.Animal reproduction · 2026Article
- A review of sperm cryopreservation in the domestic dog and cat: part I, from science to clinic.Acta veterinaria Scandinavica · 2025Review
- The role of resveratrol in male spermatogenesis: mechanisms and latest advances in clinical applications.Journal of assisted reproduction and genetics · 2025Review
- Impact of Oxidative Stress and Antioxidants on Semen Quality in Dogs.Animals : an open access journal from MDPI · 2025Review
- β-Glucan and resveratrol mitigate lead induced reproductive toxicity in male mice.Scientific reports · 2025Article
- The effect of resveratrol on the cryopreservation of Mongolian horse semen.Archives animal breeding · 2025Article
- Cryopreservation of Dog Semen.Methods in molecular biology (Clifton, N.J.) · 2025Article
- New strategies for germ cell cryopreservation: Cryoinjury modulation.Clinical and experimental reproductive medicine · 2023Article
- The Beneficial Effect of Resveratrol on the Quality of Frozen-Thawed Boar Sperm.Animals : an open access journal from MDPI · 2023Article
- Quercetin improves the apoptotic index and oxidative stress in post-thaw dog sperm.Environmental science and pollution research international · 2022Article
- Assisted reproductive techniques for canines: preservation of genetic material in domestic dogs.The Journal of reproduction and development · 2022Article
- Overview on the Antioxidants, Egg Yolk Alternatives, and Mesenchymal Stem Cells and Derivatives Used in Canine Sperm Cryopreservation.Animals : an open access journal from MDPI · 2021Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Antioxidants have multiple protective roles in a variety of cells and thus can be used to protect sperm against cryo-damage during freezing, which affects fertility. The antioxidant resveratrol (3,5,4-trihydroxytrans-stilbene; RSV) has been reported to protect the animal sperm during cryopreservation, including human sperm. In this study, we assessed the protective effects of RSV supplementation on dog sperm cryopreservation. Semen was collected from four dogs and the effect of different concentrations of RSV (0, 100, 200, and 400 µM) on post-thaw sperm quality was examined. After thawing, sperm motility was assessed using computer-aided sperm analysis, and the structural integrity of the plasma membrane, acrosome, and chromatin was examined. In addition, their mitochondrial activity and gene expression were also assessed. Dog sperm cryopreserved with 200 µM RSV showed significant improvement in post-thaw sperm motility and viability compared with that of the control group (P<0.05). Moreover, RSV-supplemented samples showed significantly higher numbers of sperm with an intact plasma membrane, active mitochondria, and structural integrity of acrosomes and chromatin than that of control samples (P<0.05). Furthermore, gene expression showed that RSV supplemented samples showed lower expression of pro-apoptotic (BAX), reactive oxygen species (ROS) modulator oxidative stress-related (ROMO1) and 8-oxoguanine DNA glycosylase 1 (OGG1) whereas higher expression levels of anti-apoptotic (BCL2), protamine-2 (PRM2), protamine-3 (PRM3) and sperm acrosome-associated 3 (SPACA3) genes than control. Our results suggest that RSV, at its optimum concentration, can be efficiently used as an antioxidant in the cryopreservation of dog sperm.
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