Evidence map›Paper›PMID 33893621›Full record

ArticleStem cell reviews and reports2021

Adipose Derived Mesenchymal Stem Cells from a Hypoxic Culture Reduce Cartilage Damage.

Jung-Pan Wang, Yu-Ting Liao, Szu-Hsien Wu, Hui-Kuang Huang, Po-Hsin Chou, En-Rung Chiang

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Article in Stem cell reviews and reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed, 1 pooled it
1.7field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 1 synthesis or guideline pooled it, 17 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Jung-Pan WangSchool of Medicine, Department of Surgery, National Yang Ming Chiao Tung University, Taipei, Taiwan. jpwang@vghtpe.gov.tw.ORCID 0000-0002-8427-9963
Yu-Ting LiaoDepartment of Orthopaedics & Traumatology, Taipei Veterans General Hospital, Taipei, Taiwan.
Szu-Hsien WuSchool of Medicine, Department of Surgery, National Yang Ming Chiao Tung University, Taipei, Taiwan.
Hui-Kuang HuangSchool of Medicine, Department of Surgery, National Yang Ming Chiao Tung University, Taipei, Taiwan.
Po-Hsin ChouDepartment of Orthopaedics & Traumatology, Taipei Veterans General Hospital, Taipei, Taiwan.
En-Rung ChiangSchool of Medicine, Department of Surgery, National Yang Ming Chiao Tung University, Taipei, Taiwan.
National Yang Ming Chiao Tung University · TWTaipei Veterans General Hospital · TWChung Hwa University of Medical Technology · TW

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The method to benifit tissue engineering of adipose-derived stem cells (ADSCs) to cartilage has been an objective of intense research in treating increasing cartilage-related disease. In this study, whether hypoxic expansion would enhance the proliferation and in vitro chondrogenic differentiation of ADSCs was studied, and then hypoxic expansion was applied to reduce cartilage damage in a rat model in vivo. Hypoxic expansion increased the proliferation and decreased the expression of aging-related genes, including p16, p21, and p53, of human ADSCs in comparison with normoxic expansion. In addition, the γH2AX expression was reduced in the hypoxic ADSCs. The chondrogenic markers were enhanced in the hypoxic ADSC differentiated chondrogenic pellets, including SOX9 on day 7 and gene expressions of COL 2 and COL 10 on day 21. To determine the in vitro chondrogenic differentiation potential of ADSCs, ADSC differentiated 21-day chondrogenic pellets were stained by Alcian blue staining and the immunostaining of COL 2 and COL 10, the results of which confirmed the enhancement of differentiation potential after the hypoxic expansion. Moreover, cartilage injury in a rat model was reduced by hypoxic ADSC treatment that was determined by histological and immunohistochemical staining detections. The effects of hypoxic expansion of ADSCs and bone marrow-derived stem cells (BMSCs) on chondrogenic differentiation potential were also compared. Smaller sizes were presented in the in vitro hypoxic BMSC differentiated chondrogenic pellets, whereas the chondrogenic marker expressions were significantly higher than those of the hypoxic ADSCs. However, there was no significant difference between the treatments of the hypoxic ADSCs and BMSCs in the cartilage injury in vivo. In conclusion, hypoxic expansion increases the chondrogenic differentiation potential of ADSCs and BMSCs in vitro and enhances them to reduce cartilage damage in vivo. Although the hypoxic BMSCs showed compact chondrogenic pellet formation and higher potential of chondrogenesis, the easy access and large resources of ADSCs still uplifted the application.

Indexed as

AdipocytesMesenchymal Stem CellsAdipose TissueAnimalsCartilageCell DifferentiationChondrogenesisRatsAdipose‐derived stem cells (ADSCs)Bone marrow‐derived stem cells (BMSCs)Chondrogenic differentiation, cartilage repairHypoxic expansion

Identifiers

PMID33893621
OpenAlexW3154690486

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.