Evidence map›Paper›PMID 33893620›Full record

ArticleStem cell reviews and reports2021

Three-dimensional Spheroid Culture Enhances Multipotent Differentiation and Stemness Capacities of Human Dental Pulp-derived Mesenchymal Stem Cells by Modulating MAPK and NF-kB Signaling Pathways.

Ya-Hui Chan, Yu-Chieh Lee, Chia-Yi Hung, Pi-Ju Yang, Pin-Chuang Lai, Sheng-Wei Feng

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Article in Stem cell reviews and reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
35citing papers in PubMed, 2 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

35 citing papers in PubMed, 2 syntheses or guidelines pooled it.

  1. Pooled it
  2. Magnetic-Based Human Tissue 3D Cell Culture: A Systematic Review.International journal of molecular sciences · 2022
    Pooled it
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Ya-Hui Chan *School of Oral Hygiene, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan.
Yu-Chieh Lee *Department of Obstetrics and Gynecology, Taipei Medical University Hospital, Taipei, Taiwan.
Chia-Yi HungSchool of Dentistry, College of Oral Medicine, Taipei Medical University, No. 250, Wuxing St, Taipei, 11031, Taiwan.
Pi-Ju YangGraduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei, Taiwan.
Pin-Chuang LaiDepartment of Diagnosis and Oral Health, School of Dentistry, University of Louisville, Louisville, KY, USA.
Sheng-Wei FengSchool of Oral Hygiene, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan. shengwei@tmu.edu.tw.ORCID 0000-0001-6637-7044

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThree-dimensional (3D) culture of mesenchymal stem cells has become an important research and development topic. However, comprehensive analysis of human dental pulp-derived mesenchymal stem cells (DPSCs) in 3D-spheroid culture remains unexplored. Thus, we evaluated the cellular characteristics, multipotent differentiation, gene expression, and related-signal transduction pathways of DPSCs in 3D-spheroid culture via magnetic levitation (3DM), compared with 2D-monolayer (2D) and 3D-aggregate (3D) cultures.

methodsThe gross morphology and cellular ultrastructure were observed in the 2D, 3D, and 3DM experimental groups using scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Surface markers and trilineage differentiation were evaluated using flow cytometry and staining analysis. Quantitative reverse transcription-polymerase chain reaction and immunofluorescence staining (IF) were performed to investigate the expression of differentiation and stemness markers. Signaling transduction pathways were evaluated using western blot analysis.

resultsThe morphology of cell aggregates and spheroids was largely influenced by the types of cell culture plates and initial cell seeding density. SEM and TEM experiments confirmed that the solid and firm structure of spheroids was quickly formed in the 3DM-medium without damaging cells. In addition, these three groups all expressed multilineage differentiation capabilities and surface marker expression. The trilineage differentiation capacities of the 3DM-group were significantly superior to the 2D and 3D-groups. The osteogenesis, angiogenesis, adipogenesis, and stemness-related genes were significantly enhanced in the 3D and 3DM-groups. The IF analysis showed that the extracellular matrix expression, osteogenesis, and angiogenesis proteins of the 3DM-group were significantly higher than those in the 2D and 3D-groups. Finally, 3DM-culture significantly activated the MAPK and NF-kB signaling transduction pathways and ameliorated the apoptosis effects of 3D-culture.

conclusionsThis study confirmed that 3DM-spheroids efficiently enhanced the therapeutic efficiency of DPSCs.

Indexed as

Mesenchymal Stem CellsCell DifferentiationDental PulpHumansNF-kappa BSignal TransductionNF-kappa B3DM-spheroid cultureCell differentiationGene expressionMAPKMesenchymal stem cellsNF-kBSignaling

Identifiers

PMID33893620

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.