ArticleScientific reports2021
Comprehensive coverage of human last meal components revealed by a forensic DNA metabarcoding approach.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Grocery intervention and DNA-based assessment to improve diet quality in pediatric obesity: a pilot randomized controlled study.Obesity (Silver Spring, Md.) · 2025Trial
- Investigating Anthropogenic and Social Influences on Diet of Semi-Urban Vervet Monkeys Using DNA Metabarcoding.Ecology and evolution · 2026Article
- Navigating Methodological Trade-Offs in eDNA Metabarcoding Biodiversity Monitoring: Insights From a Mediterranean Watershed.Molecular ecology resources · 2025Article
- Article
- Forensic species identification: practical guide for animal and plant DNA analysis.International journal of legal medicine · 2024Article
- Metaproteomics and DNA metabarcoding as tools to assess dietary intake in humans.bioRxiv : the preprint server for biology · 2024Article
- Diversity of plant DNA in stool is linked to dietary quality, age, and household income.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Article
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7 authors.
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Abstract
Stomach content analyses are a valuable tool in human forensic science to interpret perimortem events. While the identification of food components of plant and animal origin has traditionally been conducted by macro- and microscopical approaches in case of incomplete digestion, molecular methods provide the potential to increase sensitivity and taxonomic resolution. In particular, DNA metabarcoding (PCR-amplification and next generation sequencing of complex DNA mixtures) has seen a rapid growth in the field of wildlife ecology to assess species' diets from faecal and gastric samples. Despite clear advantages, molecular approaches have not yet been established in routine human forensics to investigate the last meal components of deceased persons. In this pilot study we applied for the first time a DNA metabarcoding approach to assess both plant and vertebrate components of 48 human stomach content samples taken during medicolegal autopsies. We obtained a final dataset with 34 vertebrate and 124 vegetal unique sequences, that were clustered to 9 and 33 operational taxonomic units (OTUs), respectively. Our results suggest that this approach can provide crucial information about circumstances preceding death, and open promising perspectives for biomedical dietary surveys based on digested food items found in the gastrointestinal tract.
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