ArticleNature communications2021
Cryopreservation method for Drosophila melanogaster embryos.
Article in Nature communications, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed.
- Cryopreservation and other preservation approaches for animal models workshop session I: Invertebrate models in biomedical research.Cryobiology · 2026Article
- Automated image analysis for intracellular ice detection in monolayers by freeze-substitution.Npj imaging · 2026Article
- Caenorhabditis elegans as an experimental model for resilience-boosting microbiota interventions in nonmodel ectotherms.FEMS microbiology reviews · 2026Review
- Conservation of Honeybee (Apis mellifera) Intraspecific Diversity Through Reproductive Science and Technology.Advances in experimental medicine and biology · 2026Review
- Cryopreservation of juvenile Mytilus galloprovincialis to safeguard mollusk biodiversity and support aquaculture.Scientific reports · 2025Article
- Overcoming ice: cutting-edge materials and advanced strategies for effective cryopreservation of biosample.Journal of nanobiotechnology · 2025Review
- An isochoric optical platform for interrogation of aqueous glass formation processes.RSC advances · 2024Article
- Permeability and Toxicity of Cryoprotective Agents in Silkworm Embryos: Impact on Cryopreservation.International journal of molecular sciences · 2024Article
- Ice formation and its elimination in cryopreservation of oocytes.Scientific reports · 2024Article
- 3D Printed Organisms Enabled by Aspiration-Assisted Adaptive Strategies.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Ice formation and its elimination in cryopreservation of oocytes.Research square · 2024Article
- High-throughput genetic manipulation of multicellular organisms using a machine-vision guided embryonic microinjection robot.Genetics · 2024Article
- Biopreserving Pathogens: Promise & Peril.The Journal of law, medicine & ethics : a journal of the American Society of Law, Medicine & Ethics · 2024Article
- Conduction-Dominated Cryomesh for Organism Vitrification.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Zebrafish as a high throughput model for organ preservation and transplantation research.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2023Article
- Cryopreservation and revival of Hawaiian stony corals using isochoric vitrification.Nature communications · 2023Article
- Sound waves for solving the problem of recrystallization in cryopreservation.Scientific reports · 2023Article
- An Effective Chemical Permeabilization of Silkworm Embryos.Bioengineering (Basel, Switzerland) · 2023Article
- Rapid joule heating improves vitrification based cryopreservation.Nature communications · 2022Article
- Small-molecule fulvic acid with strong hydration ability for non-vitreous cellular cryopreservation.iScience · 2022Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
The development of a widely adopted cryopreservation method remains a major challenge in Drosophila research. Here we report a robust and easily implemented cryopreservation protocol of Drosophila melanogaster embryos. We present innovations for embryo permeabilization, cryoprotectant agent loading, and rewarming. We show that the protocol is broadly applicable, successfully implemented in 25 distinct strains from different sources. We demonstrate that for most strains, >50% embryos hatch and >25% of the resulting larvae develop into adults after cryopreservation. We determine that survival can be significantly improved by outcrossing to mitigate the effect of genetic background for strains with low survival after cryopreservation. We show that flies retain normal sex ratio, fertility, and original mutation after successive cryopreservation of 5 generations and 6-month storage in liquid nitrogen. Lastly, we find that non-specialists are able to use this protocol to obtain consistent results, demonstrating potential for wide adoption.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.