ArticleScience advances2021
Spatially resolved cell polarity proteomics of a human epiblast model.
Article in Science advances, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
20 citing papers in PubMed, 31 citations in OpenAlex.
- The endo-lysosomal system drives lumen formation in a human epiblast model.Nature communications · 2026Article
- Bulk delivery of a preassembled apical surface initiates epithelial lumen formation.Nature communications · 2026Article
- Scalable synthetic peptide hydrogel enables self-organized luminal cavity architecture within hiPSC 3D colonies supporting functional hepatocyte differentiation.Bioactive materials · 2026Article
- Decoding the Apical-Basal Surfaceome of Colon Epithelial Cells via Side-Selective Biotinylation.Biomolecules · 2026Article
- The proteomic landscape and temporal dynamics of human and mouse gastruloid development.Nature cell biology · 2026Article
- High resolution spatial transcriptomic and proteomic profiling of early primate gastrulationbioRxiv : the preprint server for biology · 2026Article
- CLDN10-driven lineage decision in an amnion and primordial germ cell progenitor at the amnion-epiblast boundary in primates.Genome biology · 2025Article
- Primate amnion development.Development (Cambridge, England) · 2024Review
- Temporally resolved single cell transcriptomics in a human model of amniogenesis.bioRxiv : the preprint server for biology · 2024Article
- Article
- Lumen expansion is initially driven by apical actin polymerization followed by osmotic pressure in a human epiblast model.Cell stem cell · 2024Article
- Temporally resolved early BMP-driven transcriptional cascade during human amnion specification.bioRxiv : the preprint server for biology · 2024Article
- Monitoring Effects of Membrane Traffic Via Changes in Cell Polarity and Morphogenesis in Three-Dimensional Human Pluripotent Stem Cell Cysts.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Human epiblast lumenogenesis: From a cell aggregate to a lumenal cyst.Seminars in cell & developmental biology · 2022Review
- Excavating proteoglycan structure-function relationships: modern approaches to capture the interactions of ancient biomolecules.American journal of physiology. Cell physiology · 2022Review
- New directions for the clathrin adaptor AP-1 in cell biology and human disease.Current opinion in cell biology · 2022Review
- Determining protein polarization proteome-wide using physical dissection of individual Stentor coeruleus cells.Current biology : CB · 2022Article
- Semi-3D cultures using Laminin 221 as a coating material for human induced pluripotent stem cells.Regenerative biomaterials · 2022Article
- Proximity-Dependent Biotinylation Approaches to Explore the Dynamic Compartmentalized Proteome.Frontiers in molecular biosciences · 2022Review
- An online gathering about the latest on molecular membrane biology.The Journal of biological chemistry · 2021Article
Corrections and comments
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Authors and funding
15 authors at 3 institutions in 2 countries.
Funding
Abstract
Critical early steps in human embryonic development include polarization of the inner cell mass, followed by formation of an expanded lumen that will become the epiblast cavity. Recently described three-dimensional (3D) human pluripotent stem cell-derived cyst (hPSC-cyst) structures can replicate these processes. To gain mechanistic insights into the poorly understood machinery involved in epiblast cavity formation, we interrogated the proteomes of apical and basolateral membrane territories in 3D human hPSC-cysts. APEX2-based proximity bioinylation, followed by quantitative mass spectrometry, revealed a variety of proteins without previous annotation to specific membrane subdomains. Functional experiments validated the requirement for several apically enriched proteins in cyst morphogenesis. In particular, we found a key role for the AP-1 clathrin adaptor complex in expanding the apical membrane domains during lumen establishment. These findings highlight the robust power of this proximity labeling approach for discovering novel regulators of epithelial morphogenesis in 3D stem cell-based models.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.