ArticleStem cell reports2021
Gastruloids generated without exogenous Wnt activation develop anterior neural tissues.
Article in Stem cell reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed.
- Retinoic acid and FGF signaling interact to control elongation and lineage specification in a mouse gastruloid model.Development (Cambridge, England) · 2026Article
- Investigation of embryogenesis in a dish: the development and application of gastruloid models from pluripotent stem cells.Cell & bioscience · 2026Review
- Retinoic Acid Receptor γ Activity Plays a Critical Role in Regulating Early Mouse Gastruloid Development.International journal of molecular sciences · 2026Article
- Organizers in a dish: Modeling human CNS morphogenesis.Developmental cell · 2026Review
- Modeling Post-Implantation Mammalian Embryogenesis Using AdvancedInternational journal of molecular sciences · 2026Review
- A bioengineering approach to guide non-integrated stem cell-derived embryo model towards yolk sac development and hematopoiesis.Frontiers in cell and developmental biology · 2026Article
- Human stem cell-based embryo models: from self-organization to recapitulating development.Frontiers in cell and developmental biology · 2026Review
- Non-cell-autonomous control of mouse gastruloid development by the ultra-conserved lncRNA T-UCstem1.The EMBO journal · 2025Article
- Dual-patterned pluripotent stem cells self-organize into a human embryo model with extended anterior-posterior patterning.bioRxiv : the preprint server for biology · 2025Article
- Matrigel inhibits elongation and drives endoderm differentiation in aggregates of mouse embryonic stem cells.FEBS open bio · 2025Article
- Advances in engineered models of peri-gastrulation.iScience · 2025Review
- Stem cell culture conditions affect in vitro differentiation potential and mouse gastruloid formation.PloS one · 2025Article
- Three-dimensional stem cell models of mammalian gastrulation.BioEssays : news and reviews in molecular, cellular and developmental biology · 2024Review
- Polarization of organoids by bioengineered symmetry breaking.IBRO neuroscience reports · 2024Article
- Early autonomous patterning of the anteroposterior axis in gastruloids.Development (Cambridge, England) · 2024Article
- Article
- Topical section: embryonic models (2023) for Current Opinion in Genetics & Development.Current opinion in genetics & development · 2024Review
- Automated, High-Throughput Phenotypic Screening and Analysis Platform to Study Pre- and Post-Implantation Morphogenesis in Stem Cell-Derived Embryo-Like Structures.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Gastruloids - a minimalistic model to study complex developmental metabolism.Emerging topics in life sciences · 2023Review
- Haematopoietic development and HSC formation in vitro: promise and limitations of gastruloid models.Emerging topics in life sciences · 2023Review
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
When stimulated with a pulse from an exogenous WNT pathway activator, small aggregates of mouse embryonic stem cells (ESCs) can undergo embryo-like axial morphogenesis and patterning along the three major body axes. However, these structures, called gastruloids, currently lack the anterior embryonic regions, such as those belonging to the brain. Here, we describe an approach to generate gastruloids that have a more complete antero-posterior development. We used hydrogel microwell arrays to promote the robust derivation of mouse ESCs into post-implantation epiblast-like (EPI) aggregates in a reproducible and scalable manner. These EPI aggregates break symmetry and axially elongate without external chemical stimulation. Inhibition of WNT signaling in early stages of development leads to the formation of gastruloids with anterior neural tissues. Thus, we provide a new tool to study the development of the mouse after implantation in vitro, especially the formation of anterior neural regions.
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