Evidence map›Paper›PMID 33891176›Full record

ArticleMikrochimica acta2021

Ultrasensitive DNA methyltransferase activity sensing and inhibitor evaluation with highly photostable upconversion nanoparticle transducer.

Mingmin Wu, Mengtian Zhang, Zhiwei Fan, Xinyue Qin, Xiaoxia Zhu, Haiwei Ji, Yuling Qin, Qi Wang, Li Wu

Abstract read
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In one paragraph

Article in Mikrochimica acta, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.8field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 9 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Mingmin WuSchool of Public Health, Nantong University, Nantong, 226019, China.
Mengtian ZhangSchool of Chemistry and Materials Science, Nanjing Normal University, Nanjing, 213023, China.
Zhiwei FanMedical School, Nantong University, Nantong, 226019, China.
Xinyue QinSchool of Public Health, Nantong University, Nantong, 226019, China.
Xiaoxia ZhuSchool of Public Health, Nantong University, Nantong, 226019, China.
Haiwei JiSchool of Public Health, Nantong University, Nantong, 226019, China.
Yuling QinSchool of Public Health, Nantong University, Nantong, 226019, China.
Qi WangSchool of Public Health, Nantong University, Nantong, 226019, China. wangqi@ntu.edu.cn.
Li WuSchool of Public Health, Nantong University, Nantong, 226019, China. wuli8686@ntu.edu.cn.ORCID 0000-0002-4118-7970
Nantong University · CNNanjing Normal University · CN

Funding

Jiangsu Specially-Appointed Professor 06200048Jiangsu Specially-Appointed Professor 06200053National Natural Science Foundation of China 21877083National Natural Science Foundation of China 31901045National Natural Science Foundation of China 31901056
6 · The paper itself

Abstract

Sensitive and accurate detection of DNA methyltransferase (MTase) is conducive to the understanding of the fundamental biological processes related to DNA methylation, clinical disease diagnosis, and drug discovery. Herein, a new fluorescence transducer based on Förster resonance energy transfer (FRET) between the donor upconversion nanoparticles (UCNPs) and the efficient acceptor gold nanorods (AuNRs) for MTase activity analysis and its inhibitor screening is presented. A double-strand DNA linker between UCNPs and AuNRs could be digested by restriction endonuclease HhaI, preventing the FRET process and recovering the upconversion luminescence (UCL) intensity. With the treatment of MTase, the cutting site was disturbed by the methylation of cytosine, blocking the enzyme digestion. The transducer presented here showed an excellent analytical performance toward MTase M.HhaI in the concentration range 0.08~24 U mL

Indexed as

Enzyme InhibitorsFluorescence Resonance Energy TransferGoldLimit of DetectionBiosensing TechniquesDNA Modification MethylasesHumansMetal NanoparticlesNanotubesDNA Modification MethylasesEnzyme InhibitorsGoldDNA methyltransferase activityFRET transducerGold nanorodMethyltransferase inhibitorRestriction endonucleaseUpconversion nanoparticle

Identifiers

PMID33891176
OpenAlexW3153516932

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.