ArticleJournal of materials science. Materials in medicine2021
Optimization of decellularized human placental macroporous scaffolds for spermatogonial stem cells homing.
Article in Journal of materials science. Materials in medicine, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 34 citations in OpenAlex.
- Comparative Analysis of Decellularization Protocols for Human Placenta: Balancing Cell Removal with Extracellular Matrix Preservation for Hydrogel Applications.Annals of biomedical engineering · 2026Article
- Pancreatic tissue engineering: towards a vascularized bioengineered cure for diabetes.Frontiers in transplantation · 2026Review
- A Comparative Analysis of Culture Systems with Human Amniotic Mesenchymal Stem Cells.Journal of reproduction & infertility · 2025Article
- The revolutionary role of placental derivatives in biomedical research.Bioactive materials · 2025Review
- Testicular Cell-Conditioned Chitosan Thermosensitive Hydrogel as an Excellent Substrate for Culture and Proliferation of Spermatogonia Cells.BioMed research international · 2025Article
- Optimizations of Placenta Extracellular Matrix-Loaded Silk Fibroin/Alginate 3D-Printed Scaffolds Structurally and Functionally for Bone Tissue Engineering.Engineering in life sciences · 2025Article
- Bioengineering Approaches for Male Infertility: From Microenvironmental Regeneration to in vitro Fertilization.Advances in experimental medicine and biology · 2025Review
- Acellular porcine placental membranes as a novel biomaterial for tissue repair applications.Frontiers in bioengineering and biotechnology · 2025Article
- Testicular extracellular matrix/gelatin-based scaffold using gas foaming to support spermatogonial stem cells.Iranian journal of basic medical sciences · 2025Article
- The Decellularized Calf Testis: Introducing Suitable Scaffolds for Spermatogenesis Studies.International journal of fertility & sterility · 2023Article
- Article
- Placental-Derived Biomaterials and Their Application to Wound Healing: A Review.Bioengineering (Basel, Switzerland) · 2023Review
- 3D-printed placental-derived bioinks for skin tissue regeneration with improved angiogenesis and wound healing properties.Materials today. Bio · 2023Article
- Pipeline for precise insoluble matrisome coverage in tissue extracellular matrices.Frontiers in bioengineering and biotechnology · 2023Article
- Future Challenges and Opportunities of Extracellular Matrix Hydrogels in Female Reproductive Medicine.International journal of molecular sciences · 2022Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
Decellularized scaffolds have been found to be excellent platforms for tissue engineering applications. The attempts are still being made to optimize a decellularization protocol with successful removal of the cells with minimal damages to extracellular matrix components. We examined twelve decellularization procedures using different concentrations of Sodium dodecyl sulfate and Triton X-100 (alone or in combination), and incubation time points of 15 or 30 min. Then, the potential of the decellularized scaffold as a three-dimensional substrate for colony formation capacity of mouse spermatogonial stem cells was determined. The morphological, degradation, biocompatibility, and swelling properties of the samples were fully characterized. The 0.5%/30 SDS/Triton showed optimal decellularization with minimal negative effects on ECM (P ≤ 0.05). The swelling ratios increased with the increase of SDS and Triton concentration and incubation time. Only 0.5%/15 and 30 SDS showed a significant decrease in the SSCs viability compared with other groups (P < 0.05). The SSCs colony formation was clearly observed under SEM and H&E stained slides. The cells infiltrated into the subcutaneously implanted scaffold at days 7 and 30 post-implantation with no sign of graft rejection. Our data suggest the %0.5/30 SDS/Triton as an excellent platform for tissue engineering and reproductive biology applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.