ArticleFEMS yeast research2021
Identification of novel pentose transporters in Kluyveromyces marxianus using a new screening platform.
Article in FEMS yeast research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Article
- Characterization of three glycerol transporters with broad sugar specificities from Aspergillus niger.Applied microbiology and biotechnology · 2026Article
- Reprogramming a Hgt-family Kluyveromyces marxianus sugar transporter by site-directed mutagenesis to enable co-consumption of glucose and xylose.FEMS yeast research · 2026Article
- Article
- Evolution and functional diversification of yeast sugar transporters.Essays in biochemistry · 2023Article
- The novel properties of Kluyveromyces marxianus glucose sensor/receptor repressor pathway and the construction of glucose repression-released strains.Microbial cell factories · 2023Article
- Machine learning and comparative genomics approaches for the discovery of xylose transporters in yeast.Biotechnology for biofuels and bioproducts · 2022Article
- Identification of a novel gene required for competitive growth at high temperature in the thermotolerant yeastMicrobiology (Reading, England) · 2022Article
- Protocols for marker-free gene knock-out and knock-down in Kluyveromyces marxianus using CRISPR/Cas9.FEMS yeast research · 2022Article
- Biotechnological Importance ofJournal of fungi (Basel, Switzerland) · 2021Review
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Authors and funding
4 authors.
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Abstract
The capacity of yeasts to assimilate xylose or arabinose is strongly dependent on plasma membrane transport proteins. Because pentoses comprise a substantial proportion of available sugars in lignocellulosic hydrolysates, their utilisation is centrally important for the development of second generation biorefineries. Relatively few native pentose transporters have been studied and there is intense interest in expanding the repertoire. To aid the identification of novel transporters, we developed a screening platform in the native pentose-utilising yeast Kluyveromyces marxianus. This involved the targeted deletion of twelve transporters of the major facilitator superfamily (MFS) and application of a synthetic biology pipeline for rapid testing of candidate pentose transporters. Using this K. marxianus ΔPT platform, we identified several K. marxianus putative xylose or arabinose transporter proteins that recovered a null strain's ability to growth on these pentoses. Four proteins of the HGT-family were able to support growth in media with high or low concentrations of either xylose or arabinose, while six HXT-like proteins displayed growth only at high xylose concentrations, indicating solely low affinity transport activity. The study offers new insights into the evolution of sugar transporters in yeast and expands the set of native pentose transporters for future functional and biotechnological studies.
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