Evidence map›Paper›PMID 33889664›Full record

ArticleBio-protocol2021

FRET-based Microscopy Assay to Measure Activity of Membrane Amino Acid Transporters with Single-transporter Resolution.

Didar Ciftci, Gerard H M Huysmans, Xiaoyu Wang, Changhao He, Daniel Terry, Zhou Zhou, Gabriel Fitzgerald, Scott C Blanchard, Olga Boudker

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bio-protocol, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.4field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 3 citations in OpenAlex.

  1. Multimodal dynamics control activity of a glial glutamate transporter.bioRxiv : the preprint server for biology · 2026
    Article
  2. Article
  3. Article
  4. Structural basis of pH-dependent activation in a CLC transporter.Nature structural & molecular biology · 2024
    Article
  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Didar CiftciDepartment of Physiology and Biophysics, Weill Cornell Medicine, New York, USA.
Gerard H M HuysmansDepartment of Physiology and Biophysics, Weill Cornell Medicine, New York, USA.
Xiaoyu WangDepartment of Physiology and Biophysics, Weill Cornell Medicine, New York, USA.
Changhao HeDepartment of Physiology and Biophysics, Weill Cornell Medicine, New York, USA.
Daniel TerryDepartment of Physiology and Biophysics, Weill Cornell Medicine, New York, USA.
Zhou ZhouDepartment of Physiology and Biophysics, Weill Cornell Medicine, New York, USA.
Gabriel FitzgeraldDepartment of Physiology and Biophysics, Weill Cornell Medicine, New York, USA.
Scott C BlanchardDepartment of Physiology and Biophysics, Weill Cornell Medicine, New York, USA.
Olga BoudkerDepartment of Physiology and Biophysics, Weill Cornell Medicine, New York, USA.
Weill Cornell Medicine · USCornell University · US

Funding

The structural dynamics of a glutamate transporter homologueR37NS085318 · NINDS · WEILL MEDICAL COLL OF CORNELL UNIV · PI BOUDKER, OLGA · 2014 to 2020
$4.3M
Next-generation Fluorescent Probes for Biological ResearchR01GM098859 · NIGMS · WEILL MEDICAL COLL OF CORNELL UNIV · PI BLANCHARD, SCOTT C · 2012 to 2019
$2.9M
NIGMS NIH HHS R01 GM098859NINDS NIH HHS R37 NS085318
6 · The paper itself

Abstract

Secondary active transporters reside in cell membranes transporting polar solutes like amino acids against steep concentration gradients, using electrochemical gradients of ions as energy sources. Commonly, ensemble-based measurements of radiolabeled substrate uptakes or transport currents inform on kinetic parameters of transporters. Here we describe a fluorescence-based functional assay for glutamate and aspartate transporters that provides single-transporter, single-transport cycle resolution using an archaeal elevator-type sodium and aspartate symporter Glt

Indexed as

Amino-acid sensorGlutamate transporterSingle-molecule FRETTIRF microscopyTransport assay

Identifiers

PMID33889664
PMCPMC8054209
OpenAlexW3152466469

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.