ArticleJournal of applied oral science : revista FOB2021
The effect of reduced glutathione on the toxicity of silver diamine fluoride in rat pulpal cells.
Article in Journal of applied oral science : revista FOB, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 29 citations in OpenAlex.
- Comparative Cytocompatibility and Oxidative Stress Analysis of Green-Synthesized Nano-Silver Fluoride and Silver Diamine Fluoride in Human Gingival Fibroblasts.Journal of functional biomaterials · 2026Article
- Effect of nano silver fluoride pre-treatment on the microtensile bond strength of a universal adhesive to dentin: an in vitro study.Biomaterial investigations in dentistry · 2026Article
- Pulpal response following SDF application for deep carious lesions:AnBioinformation · 2026Article
- Effect of single and repeated potassium iodide applications versus glutathione on silver diamine fluoride-induced tooth discoloration in primary teeth: An in vitro comparative study.Journal of dental research, dental clinics, dental prospects · 2025Article
- Evaluation of the cytotoxicity of new formulations of cariostatic agents containing nano silver fluoride: an in vitro study.Odontology · 2025Article
- Effects of Capsaicin on Migration and Alkaline Phosphatase Activity of Dental Pulp Cells.European journal of dentistry · 2024Article
- Article
- Article
- Comparing cytocompatibility of two fluoride-containing solutions and two resin-based restorative materials-a pilot study.Frontiers in oral health · 2024Article
- Clinical and primary evidence of silver diamine fluoride on root caries management.The Japanese dental science review · 2022Review
- Radiographic Changes to Silver Diamine Fluoride Treated Carious Lesions after a Rinsing Step.Dentistry journal · 2022Article
- Characterization of Physical and Biological Properties of a Caries-Arresting Liquid Containing Copper Doped Bioglass Nanoparticles.Pharmaceutics · 2022Article
- The Cytocompatibility of Silver Diamine Fluoride on Mesenchymal Stromal Cells from Human Exfoliated Deciduous Teeth: An In Vitro Study.Materials (Basel, Switzerland) · 2022Article
- Effects of Silver Diamine Nitrate and Silver Diamine Fluoride on Dentin Remineralization and Cytotoxicity to Dental Pulp Cells: An In Vitro Study.Journal of functional biomaterials · 2022Article
- Effects of Different Application Times of Silver Diamine Fluoride on Mineral Precipitation in Demineralized Dentin.Dentistry journal · 2021Article
- Penetration of Silver Diamine Fluoride in Deep Carious Lesions of Human Permanent Teeth: An In Vitro Study.International journal of dentistry · 2021Article
- The cytotoxic and biological effects of silver diamine fluoride on human dental pulp cells: A systematic review and meta-analysis.Journal of oral biology and craniofacial researchReview
- Evaluation of cytotoxicity of 3.8 % SDF and BioAKT solutions on the viability of dental pulp stem cells.Journal of oral biology and craniofacial researchArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionDue to its ability to arrest untreated dental caries, silver diamine fluoride (SDF) has been advocated for indirect pulp capping procedures. However, the high concentrations of silver and fluoride in SDF raise concerns about its biocompatibility to pulpal tissues.
objectivesThis study aimed to investigate the effect of SDF on the viability, alkaline phosphatase (ALP) activity, and morphology of pulpal-like cells (RPC-C2A) and to evaluate the influence of reduced glutathione (GSH) on SDF-induced cytotoxicity and deposit formation on dentin. METHODOLOGY: The cytotoxicity of diluted 38% SDF solutions (10-4 and 10-5), with or without the addition of 5 mM or 50 mM GSH, was evaluated at 6 and 24 hours. Cell viability was detected using WST-8 and the effect on ALP activity was performed using an ALP assay kit. Cell morphology was observed using a phase-contrast microscope. Scanning electron microscopy analysis was conducted to evaluate the effect of GSH incorporation or conditioning on SDF-induced deposit formation on dentin discs. Cytotoxicity data were analyzed by two-way analysis of variance (ANOVA) and Tukey post hoc tests (p<0.05).
resultsThere were significant differences between the groups. The results demonstrated that all tested SDF dilutions caused a remarkable cytotoxic effect, while the addition of GSH prevented SDF-induced damage at 6-hour exposure time in the higher dilution of SDF. Dentin treated with plain SDF or GSH-incorporated SDF solution showed deposit formation with occluded dentinal tubules, unlike the other groups.
conclusionSDF severely disturbed the viability, mineralization-ability, and morphology of pulpal-like cells, while controlled concentrations of GSH had a short-term protective effect against SDF-induced damage. GSH showed an inhibitory effect on SDF-induced dentinal deposit formation. Further research is warranted to evaluate the effect of GSH on caries-arresting, anti-hypersensitivity, and antibacterial functions of SDF.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.