Evidence map›Paper›PMID 33886644›Full record

ArticlePloS one2021

Versican contributes to ligament formation of knee joints.

Tomoko Higuchi, Daisuke Suzuki, Takafumi Watanabe, Kanda Fanhchaksai, Keiko Ota, Kazuhisa Yokoo, Hiroshi Furukawa, Hideto Watanabe

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.9field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Tomoko HiguchiDepartment of Plastic Surgery, Aichi Medical University, Nagakute, Japan.
Daisuke SuzukiDepartment of Health Sciences, Hokkaido Chitose College of Rehabilitation, Chitose, Japan.
Takafumi WatanabeLaboratory of Veterinary Anatomy, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Japan.
Kanda FanhchaksaiInstitute for Molecular Science of Medicine, Aichi Medical University, Nagakute, Japan.
Keiko OtaInstitute for Molecular Science of Medicine, Aichi Medical University, Nagakute, Japan.
Kazuhisa YokooDepartment of Plastic Surgery, Aichi Medical University, Nagakute, Japan.
Hiroshi FurukawaDepartment of Plastic Surgery, Aichi Medical University, Nagakute, Japan.
Hideto WatanabeInstitute for Molecular Science of Medicine, Aichi Medical University, Nagakute, Japan.ORCID 0000-0001-5291-0696
Aichi Medical University · JPHokkaido Chitose College of Rehabilitation · JPRakuno Gakuen University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Versican is a large proteoglycan in the extracellular matrix. During embryonic stages, it plays a crucial role in the development of cartilage, heart, and dermis. Previously, we reported that Prx1-Vcan conditional knockout mice, lacking Vcan expression in mesenchymal condensation areas of the limb bud, show the impaired joint formation and delayed cartilage development. Here, we investigated their phenotype in adults and found that they develop swelling of the knee joint. Histologically, their newborn joint exhibited impaired formation of both anterior and posterior cruciate ligaments. Immunostaining revealed a decrease in scleraxis-positive cells in both articular cartilage and ligament of Prx1-Vcan knee joint, spotty patterns of type I collagen, and the presence of type II collagen concomitant with the absence of versican expression. These results suggest that versican expression during the perinatal period is required for cruciate ligaments' formation and that its depletion affects joint function in later ages.

Indexed as

AnimalsAnimals, NewbornAnterior Cruciate LigamentBasic Helix-Loop-Helix ProteinsCartilage, ArticularChondrogenesisCollagen Type ICollagen Type IIKnee JointMiceMice, KnockoutPhenotypePosterior Cruciate LigamentVersicansBasic Helix-Loop-Helix ProteinsCollagen Type ICollagen Type IIScx protein, mouseVcan protein, mouseVersicans

Identifiers

PMID33886644
PMCPMC8061984
OpenAlexW3153890652

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.