Evidence map›Paper›PMID 33886019›Full record

ArticleBiodegradation2021

Outer membrane vesicles mediated horizontal transfer of an aerobic denitrification gene between Escherichia coli.

Weichuan Qiao, Lianjie Wang, Yang Luo, Jiahui Miao

Abstract read
PubMed Publisher
In one paragraph

Article in Biodegradation, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
0.9field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 18 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
  4. Review
  5. Article
  6. Article
  7. Bacterial extracellular vesicles target different bacterial species, impairing cell division and diminishing their pathogenicity.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  8. Review
  9. Role ofFrontiers in microbiology · 2023
    Review
  10. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 2 countries.

Weichuan QiaoDepartment of Environmental Engineering, College of Biology and the Environment, Nanjing Forestry University, Nanjing, 210037, China. hgqwc@njfu.edu.cn.ORCID 0000-0001-9721-9364
Lianjie WangDepartment of Environmental Engineering, College of Biology and the Environment, Nanjing Forestry University, Nanjing, 210037, China.
Yang LuoDepartment of Environmental Engineering, College of Biology and the Environment, Nanjing Forestry University, Nanjing, 210037, China.
Jiahui MiaoDepartment of Environmental Engineering, College of Biology and the Environment, Nanjing Forestry University, Nanjing, 210037, China.
Nanjing Forestry University · CN

Funding

Natural Science Foundation of Jiangsu Province BK20201388the Industry Prospect and Common Key Technologies in Jiangsu Province BE2018015
6 · The paper itself

Abstract

Bacterial genetic material can be horizontally transferred between microorganisms via outer membrane vesicles (OMVs) released by bacteria. Up to now, the application of vesicle-mediated horizontal transfer of "degrading genes" in environmental remediation has not been reported. In this study, the nirS gene from an aerobic denitrification bacterium, Pseudomonas stutzeri, was enclosed in a pET28a plasmid, transformed into Escherichia coli (E. coli) DH5α and expressed in E. coli BL21. The E. coli DH5α released OMVs containing the recombination plasmid pET28a-nirS-EGFP. When compared with the free pET28a-nirS-EGFP plasmid's inability to transform, nirS in OMVs could be transferred into E. coli BL21 with the transformation frequency of 2.76 × 10

Indexed as

DenitrificationEscherichia coliBiodegradation, EnvironmentalPlasmidsDenitrificationHorizontal gene transferNirS geneVesicles

Identifiers

PMID33886019
OpenAlexW3156244254

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.