ArticleBiotechnology for biofuels2021
Further engineering of R. toruloides for the production of terpenes from lignocellulosic biomass.
Article in Biotechnology for biofuels, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Metabolic unlocking of lycopene accumulation in the oleaginous yeast Rhodotorula toruloides.Biotechnology letters · 2026Article
- The Mevalonate Pathway: Innovations, Applications, and Challenges in Biotechnology with Emphasis on Fungal Biology.Journal of fungi (Basel, Switzerland) · 2026Review
- Biosynthesis of patchoulol via metabolic engineering the oleaginous red yeastSynthetic and systems biotechnology · 2026Article
- Higher Wood Density Lowers Feedstock Cost and Has Minimal Impact on Biomass Conversion to Biofuels.ACS sustainable chemistry & engineering · 2026Article
- Metabolic Engineering ofJournal of fungi (Basel, Switzerland) · 2026Article
- Bioactivities and biosynthesis of monoterpene-based biopesticides: current state and perspectives.Microbial cell factories · 2026Review
- Evaluating isoprenol production using the IPP-bypass pathway in the oleaginous yeast Rhodosporidium toruloides.Biotechnology for biofuels and bioproducts · 2026Article
- Article
- Establishment of a protoplast transformation method for the oleaginous yeast Rhodotorula toruloides.FEMS yeast research · 2026Article
- Current Directions of Selected Plant-Origin Wastes' Valorization in Biotechnology of Food Additives and Other Important Chemicals.Foods (Basel, Switzerland) · 2025Review
- Biosynthesis of Edible Terpenoids: Hosts and Applications.Foods (Basel, Switzerland) · 2025Review
- Recent advances in genetic engineering and chemical production in yeast species.FEMS yeast research · 2025Review
- Ashbya gossypii as a versatile platform to produce sabinene from agro-industrial wastes.Fungal biology and biotechnology · 2024Article
- Biosynthesis Progress of High-Energy-Density Liquid Fuels Derived from Terpenes.Microorganisms · 2024Review
- Corn stover variability drives differences in bisabolene production by engineered Rhodotorula toruloides.Journal of industrial microbiology & biotechnology · 2024Article
- Metabolic engineering of terpene metabolism in lavender.Beni-Suef University journal of basic and applied sciences · 2024Review
- Article
- PeakDecoder enables machine learning-based metabolite annotation and accurate profiling in multidimensional mass spectrometry measurements.Nature communications · 2023Article
- Valorization of Hemp-Based Packaging Waste with One-Pot Ionic Liquid Technology.Molecules (Basel, Switzerland) · 2023Article
- Modularly engineeringFrontiers in bioengineering and biotechnology · 2023Article
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22 authors.
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Abstract
backgroundMitigation of climate change requires that new routes for the production of fuels and chemicals be as oil-independent as possible. The microbial conversion of lignocellulosic feedstocks into terpene-based biofuels and bioproducts represents one such route. This work builds upon previous demonstrations that the single-celled carotenogenic basidiomycete, Rhodosporidium toruloides, is a promising host for the production of terpenes from lignocellulosic hydrolysates.
resultsThis study focuses on the optimization of production of the monoterpene 1,8-cineole and the sesquiterpene α-bisabolene in R. toruloides. The α-bisabolene titer attained in R. toruloides was found to be proportional to the copy number of the bisabolene synthase (BIS) expression cassette, which in turn influenced the expression level of several native mevalonate pathway genes. The addition of more copies of BIS under a stronger promoter resulted in production of α-bisabolene at 2.2 g/L from lignocellulosic hydrolysate in a 2-L fermenter. Production of 1,8-cineole was found to be limited by availability of the precursor geranylgeranyl pyrophosphate (GPP) and expression of an appropriate GPP synthase increased the monoterpene titer fourfold to 143 mg/L at bench scale. Targeted mevalonate pathway metabolite analysis suggested that 3-hydroxy-3-methyl-glutaryl-coenzyme A reductase (HMGR), mevalonate kinase (MK) and phosphomevalonate kinase (PMK) may be pathway bottlenecks are were therefore selected as targets for overexpression. Expression of HMGR, MK, and PMK orthologs and growth in an optimized lignocellulosic hydrolysate medium increased the 1,8-cineole titer an additional tenfold to 1.4 g/L. Expression of the same mevalonate pathway genes did not have as large an impact on α-bisabolene production, although the final titer was higher at 2.6 g/L. Furthermore, mevalonate pathway intermediates accumulated in the mevalonate-engineered strains, suggesting room for further improvement.
conclusionsThis work brings R. toruloides closer to being able to make industrially relevant quantities of terpene from lignocellulosic biomass.
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