ArticleArchives of virology2021
Development and use of a reverse transcription insulated isothermal PCR assay for detection and characterization of bovine torovirus in yaks.
Article in Archives of virology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
5 citing papers in PubMed, 6 citations in OpenAlex.
- Isolation, Genomic Evolution, and Pathogenicity ofAnimals : an open access journal from MDPI · 2026Article
- Analysis for Bopivirus B in goats in the Sichuan province, China using a novel TaqMan real-time polymerase chain reaction assay.Acta veterinaria Scandinavica · 2025Article
- Epidemiological survey of calf diarrhea related viruses in several areas of Guangdong Province.Frontiers in microbiology · 2024Article
- Development of a one-step multiplex RT-qPCR method for rapid detection of bovine diarrhea viruses.Frontiers in cellular and infection microbiology · 2024Article
- A multiplex real-time fluorescence-based quantitative PCR assay for calf diarrhea viruses.Frontiers in microbiology · 2023Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Bovine torovirus (BToV) is an important diarrhea-causing pathogen affecting bovines. To facilitate BToV detection, a reverse transcription insulated isothermal PCR (RT-iiPCR) assay was developed that targets the BToV M gene with high specificity and reproducibility. The assay has a limit of detection of 23 copies/μL. Out of 69 diarrheic fecal samples from yaks collected on six farms in Tibet and Sichuan provinces in China, 11.59% (8/69) tested positive for BToV using this assay. The full-length spike (S) and hemagglutinin-esterase (HE) genes of three positive samples were subsequently sequenced. Notably, an identical recombination event was identified in the S1 subunit of the S protein of three isolates. All of the HE genes were found to belong to genotype III and shared the same unique aa variation (P44S) in the esterase domain. This study is the first confirmation of BToV in yaks and the first report of an S gene recombination event in BToV. Our findings will enhance the current understanding of the molecular characteristics and genetic evolution of BToV.
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Registered trials
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