ArticleMolecular biology of the cell2021
A yeast FRET biosensor enlightens cAMP signaling.
Article in Molecular biology of the cell, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 22 citations in OpenAlex.
- Glucose repression ofbioRxiv : the preprint server for biology · 2026Article
- The role of fungal G protein-coupled receptors in interspecies cell-cell communication.FEMS microbiology reviews · 2026Review
- A Dive Into Yeast's Sugar Diet-Comparing the Metabolic Response of Glucose, Fructose, Sucrose, and Maltose Under Dynamic Feast/Famine Conditions.Biotechnology and bioengineering · 2025Article
- Systems level analysis of time and stimuli specific signaling through PKA.Molecular biology of the cell · 2024Article
- Controlling circuitry underlies the growth optimization of Saccharomyces cerevisiae.Metabolic engineering · 2023Article
- Article
- Dynamic fluctuations in a bacterial metabolic network.Nature communications · 2023Article
- Using the AKAR3-EV biosensor to assess Sch9p- and PKA-signalling in budding yeast.FEMS yeast research · 2023Article
- SystematicACS synthetic biology · 2022Article
- Kinetic Modeling ofMetabolites · 2022Review
- Molecular Targets and Biological Functions of cAMP Signaling inBiomolecules · 2021Article
- Nutrient sensing and cAMP signaling in yeast: G-protein coupled receptor versus transceptor activation of PKA.Microbial cell (Graz, Austria) · 2020Article
- An Improved ATP FRET Sensor For Yeast Shows Heterogeneity During Nutrient Transitions.ACS sensors · 2020Article
- Synthesis of Programmable Bioelectronic Yeast for Biohybrid Futures.Engineering biologyReview
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The cAMP-PKA signaling cascade in budding yeast regulates adaptation to changing environments. We developed yEPAC, a FRET-based biosensor for cAMP measurements in yeast. We used this sensor with flow cytometry for high-throughput single cell-level quantification during dynamic changes in response to sudden nutrient transitions. We found that the characteristic cAMP peak differentiates between different carbon source transitions and is rather homogenous among single cells, especially for transitions to glucose. The peaks are mediated by a combination of extracellular sensing and intracellular metabolism. Moreover, the cAMP peak follows the Weber-Fechner law; its height scales with the relative, and not the absolute, change in glucose. Last, our results suggest that the cAMP peak height conveys information about prospective growth rates. In conclusion, our yEPAC-sensor makes possible new avenues for understanding yeast physiology, signaling, and metabolic adaptation.
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