Evidence map›Paper›PMID 33880705›Full record

ArticleJournal of fluorescence2021

Quantitative Fluorescence Resonance Energy Transfer Analysis on the Direct Interaction of Activation-2b with Histone H3/Switch-3B Protein in Arabidopsis Mesophyll Protoplasts.

Lu Gao, Fangrui Lin, Danlu Han, Jieming Jiang, Chengwei Yang, Zhengfei Zhuang, Tongsheng Chen

Abstract read
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In one paragraph

Article in Journal of fluorescence, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Lu Gao *MOE Key Laboratory of Laser Life Science & College of Life Science, South China Normal University, Guangzhou, 510631, China.
Fangrui Lin *Key Laboratory of Optoelectronic Devices and Systems of Guangdong Province & Ministry of Education, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen, 518060, Guangdong Province, China.
Danlu Han *Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Science, South China Normal University, Guangzhou, 510631, China.
Jieming JiangGuangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Science, South China Normal University, Guangzhou, 510631, China.
Chengwei YangGuangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Science, South China Normal University, Guangzhou, 510631, China.
Zhengfei ZhuangSCNU Qingyuan Institute of Science and Technology Innovation Co., Ltd., Qingyuan, 511517, China. zhuangzf@scnu.edu.cn.
Tongsheng ChenMOE Key Laboratory of Laser Life Science & College of Life Science, South China Normal University, Guangzhou, 510631, China. chentsh@scnu.edu.cn.ORCID http://orcid.org/0000-0002-9036-6676

Funding

National Natural Science Foundation of China 61527825National Natural Science Foundation of China 61875056Science and Technology Program of Guangzhou 2019050001
6 · The paper itself

Abstract

Interaction between the alteration/deficiency in activation-2b (ADA2b) and histone H3/switch-3B (SWI3B) proteins was evaluated in arabidopsis mesophyll protoplasts by quantitative fluorescence resonance energy transfer (FRET) analysis. Microscopic image showed that ADA2b, SWI3B and H3 proteins colocalized in nucleus, and quantitative FRET measurements showed 0.31 of FRET efficiency (E) for the protoplasts coexpressing ECFP-ADA2b and EYFP-SWI3B, and 0.285 of E for the protoplasts coexpressing ECFP-H3 and EYFP-ADA2b, demonstrating the direct interaction of ADA2b with SWI3B/H3 protein. Collectively, SWI3B and H3 proteins are the inherent components of the ADA2b complex in which ADA2b directly interacts with SWI3B/H3 protein.

Indexed as

ArabidopsisArabidopsis ProteinsFluorescence Resonance Energy TransferHistonesProtoplastsMesophyll CellsProtein BindingRNA-Binding ProteinsTranscription FactorsADA2b protein, ArabidopsisArabidopsis ProteinsATSWI3B protein, ArabidopsisHistonesRNA-Binding ProteinsTranscription FactorsADA2bFRETProtein interactionSWI3B

Identifiers

PMID33880705

What OpenQuestion holds

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None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.