Evidence map›Paper›PMID 33879891›Full record

ArticleBeijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences2021

[Therapeutic effect of gene silencing peptidyl arginine deaminase 4 on pulmonary interstitial lesions induced by collagen-induced arthritis mice].

K Zhao, Z F Chang, Z H Wang, C Y Pang, Y F Wang

Open access · greenAbstract read
PubMed Full text at PMC
In one paragraph

Article in Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact, top 95% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

K ZhaoDepartment of Rheumatism and Immunology, the First Affiliated Hospital of Baotou Medical College, Inner Monolia University of Secience and Technology, Inner Mongolia Autoimmunology Key Laboratory, Baotou 014010, Inner Mongolia, China.
Z F ChangDepartment of Rheumatism and Immunology, the First Affiliated Hospital of Baotou Medical College, Inner Monolia University of Secience and Technology, Inner Mongolia Autoimmunology Key Laboratory, Baotou 014010, Inner Mongolia, China.
Z H WangDepartment of Rheumatism and Immunology, the First Affiliated Hospital of Baotou Medical College, Inner Monolia University of Secience and Technology, Inner Mongolia Autoimmunology Key Laboratory, Baotou 014010, Inner Mongolia, China.
C Y PangDepartment of Rheumatism and Immunology, the First Affiliated Hospital of Baotou Medical College, Inner Monolia University of Secience and Technology, Inner Mongolia Autoimmunology Key Laboratory, Baotou 014010, Inner Mongolia, China.
Y F WangDepartment of Rheumatism and Immunology, the First Affiliated Hospital of Baotou Medical College, Inner Monolia University of Secience and Technology, Inner Mongolia Autoimmunology Key Laboratory, Baotou 014010, Inner Mongolia, China.
Inner Mongolia University of Science and Technology · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveTo investigate the therapeutic effect of gene silencing peptidyl arginine deaminase 4 (PAD4) on pulmonary interstitial lesions induced by collagen-induced arthritis (CIA) mice, and possible mechanisms.

methodsA CIA mouse model was established in DBA/1 mice, followed by a tail vein injection of the virus solution prepared by the PAD4-siRNA expression vector once a week for 8 times. The mice were sacrificed at the end of the experiment. The expression of PAD4 mRNA in lungs was detected by real-time quantitative PCR (qRT-PCR). The expression of PAD4 protein was detected by tissue immunohistochemistry. Cell culture was performed by spleen tissue. Flow cytometry changes in the ratio of Tfh cells to Tfr cells were examined; lung staining was performed in the lungs to observe changes in lung pathology.

results(1) Compared with the blank group, the expression of PAD4 mRNA in the lung tissue of the model group increased, the difference was statistically significant (

conclusionGene silencing of PAD4 can reduce the proportion of Tfh cells, increase the proportion of Tfr cells, reverse the proportion of Tfh/Tfr, and reduce the degree of interstitial lesions and inflammatory infiltration of lung tissue.

Indexed as

Arthritis, ExperimentalAnimalsArginineGene SilencingLungMiceMice, Inbred DBAArginineFollicular helper T cellsFollicular regulatory T cellGene silencingPeptidyl arginine deaminase 4Rheumatoid arthritis

Identifiers

PMID33879891
PMCPMC8072419
OpenAlexW3153294857

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.