ArticleMethods in molecular biology (Clifton, N.J.)2021
Gel-Based Analysis of Protein-Nucleic Acid Interactions.
Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 10 citations in OpenAlex.
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- Analysis of Protein-RNA Interactions by Protein-Induced Fluorescence Enhancement (PIFE).Methods in molecular biology (Clifton, N.J.) · 2026Article
- From binding to networks: methods for identifying transcription factor targets in plant systems.Frontiers in plant science · 2026Review
- FucR Functions as a Transcriptional Regulator for L-Fucose Utilization inMicroorganisms · 2025Article
- FluoTag-EMSA: a fast and accessible quantitative method to assess RNA-binding specificity using 3'-tagged hybrid duplexes.Frontiers in molecular biosciences · 2025Article
- Proteomics and acetyl-proteomics reveal the antibacterial mechanism of berberine sulfate against methicillin-resistantFrontiers in microbiology · 2025Article
- Biophysical Characterization of the MMethods in molecular biology (Clifton, N.J.) · 2025Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Electrophoretic mobility shift assays (EMSAs) are among the most frequently used and straightforward experiments for studying protein-nucleic acid interactions. EMSAs rely on the principle that protein-nucleic acid complexes have reduced electrophoretic mobility in a native gel matrix compared to free nucleic acid due to their larger size and reduced negative charge. Therefore, bands for the protein-nucleic acid complexes are shifted in a gel and can be distinguished from free nucleic acids. EMSAs remain a popular technique since they do not require specialist equipment and the complexes formed are easily visualized. Furthermore, the technique can be adapted to enable various aspects of protein-nucleic acid interactions to be investigated, including sequence specificity, estimated binding affinity, and binding stoichiometry.
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Registered trials
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