ArticleNucleic acids research2021
A ribonuclease III involved in virulence of Mucorales fungi has evolved to cut exclusively single-stranded RNA.
Article in Nucleic acids research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 17 citations in OpenAlex.
- Exosomal miRNAs in pancreatitis: Mechanisms and potential applications (Review).Molecular medicine reports · 2025Review
- A group of segmented viruses contains genome segments sharing homology with multiple viral taxa.Journal of virology · 2025Article
- Direct Targeted Degradation of Transposon RNAs by the Non-Canonical RNAi Pathway of the FungusInternational journal of molecular sciences · 2025Article
- Clade-wide fungal proteome analysis reveals structure-function conservation in divergent Dicer proteins.Computational and structural biotechnology journal · 2025Article
- Major components of RNAi gene families inHeliyon · 2024Article
- Advances in understanding infections caused by the basal fungus Mucor.PLoS pathogens · 2023Article
- Review
- RNA-based therapeutics to treat human fungal infections.Trends in microbiology · 2022Review
- Genetic Manipulation in Mucorales and New Developments to Study Mucormycosis.International journal of molecular sciences · 2022Review
- Role of the Non-Canonical RNAi Pathway in the Antifungal Resistance and Virulence of Mucorales.Genes · 2021Review
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Members of the ribonuclease III (RNase III) family regulate gene expression by processing double-stranded RNA (dsRNA). This family includes eukaryotic Dicer and Drosha enzymes that generate small dsRNAs in the RNA interference (RNAi) pathway. The fungus Mucor lusitanicus, which causes the deadly infection mucormycosis, has a complex RNAi system encompassing a non-canonical RNAi pathway (NCRIP) that regulates virulence by degrading specific mRNAs. In this pathway, Dicer function is replaced by R3B2, an atypical class I RNase III, and small single-stranded RNAs (ssRNAs) are produced instead of small dsRNA as Dicer-dependent RNAi pathways. Here, we show that R3B2 forms a homodimer that binds to ssRNA and dsRNA molecules, but exclusively cuts ssRNA, in contrast to all known RNase III. The dsRNA cleavage inability stems from its unusual RNase III domain (RIIID) because its replacement by a canonical RIIID allows dsRNA processing. A crystal structure of R3B2 RIIID resembles canonical RIIIDs, despite the low sequence conservation. However, the groove that accommodates dsRNA in canonical RNases III is narrower in the R3B2 homodimer, suggesting that this feature could be responsible for the cleavage specificity for ssRNA. Conservation of this activity in R3B2 proteins from other mucormycosis-causing Mucorales fungi indicates an early evolutionary acquisition.
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