ArticlePediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology2021
Increased miR-21-3p and miR-487b-3p serum levels during anaphylactic reaction in food allergic children.
Article in Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.
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14 citing papers in PubMed, 1 synthesis or guideline pooled it, 35 citations in OpenAlex.
- MicroRNA expression and their molecular targets in food allergies: a systematic review.Frontiers in immunology · 2025Pooled it
- The involvement of human breastmilk-derived extracellular vesicles in the development of infant atopic sensitization.Allergologie select · 2026Review
- Towards a Multi-omics Understanding of Anaphylaxis: Insights into Pathogenesis and Biomarker Identification.Clinical reviews in allergy & immunology · 2025Review
- Identifying patients at risk of anaphylaxis.The World Allergy Organization journal · 2024Review
- Study of MicroRNAs Expression in Food Allergy.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Circulating serum profile of small non-coding RNAs in patients with anaphylaxis beyond microRNAs.Frontiers in allergy · 2024Article
- miRNA 548a-3p as biomarker of NEDA-3 at 2 years in multiple sclerosis patients treated with fingolimod.Journal of neuroinflammation · 2023Article
- Novel mediator in anaphylaxis: decreased levels of miR-375-3p in serum and within extracellular vesicles of patients.Frontiers in immunology · 2023Article
- An interdisciplinary approach to characterize peanut-allergic patients-First data from the FOOD@ consortium.Clinical and translational allergy · 2022Article
- Serological profiling reveals hsa-miR-451a as a possible biomarker of anaphylaxis.JCI insight · 2022Article
- Pathophysiological, Cellular, and Molecular Events of the Vascular System in Anaphylaxis.Frontiers in immunology · 2022Review
- Genes involved in platelet aggregation and activation are downregulated during acute anaphylaxis in humans.Clinical & translational immunology · 2022Article
- State-of-the-Art on Biomarkers for Anaphylaxis in Obstetrics.Life (Basel, Switzerland) · 2021Review
- Increased miR-21-3p and miR-487b-3p serum levels during anaphylactic reaction in food allergic children.Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology · 2021Article
Corrections and comments
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Authors and funding
15 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAnaphylaxis is the most severe manifestation of allergic disorders. The poor knowledge of its molecular mechanisms often leads to under-diagnosis. MicroRNAs (miRNA) regulate physiologic and pathologic processes, and they have been postulated as promising diagnostic markers. The main objectives of this study were to characterize the human miRNA profile during anaphylaxis and to assess their capacity as diagnostic markers and determine their participation in the molecular mechanisms of this event.
methodsThe miRNA serum profiles from the acute and baseline phase of 5 oral food-challenged anaphylactic children (<18 years old) were obtained by next-generation sequencing (NGS). From the panel of statistically significant miRNAs obtained, several candidates were selected and analyzed in 19 anaphylactic children by qPCR. We performed system biology analysis (SBA) on their target genes to identify main functions and canonical pathways. A functional in vitro assay was carried out incubating endothelial cells (ECs) in anaphylactic conditions.
resultsThe NGS identified 389 miRNAs among which 41 were significantly different between acute and baseline samples. The high levels of miR-21-3p (fold change = 2.28, P = .006) and miR-487b-3p (fold change = 1.04, P = .039) observed by NGS in acute serum samples were confirmed in a larger group of 19 patients. The SBA revealed molecular pathways related to the inflammation and immune system regulation. miR-21-3p increased intracellularly and in acute phase serum after EC stimulation.
conclusionsThese findings provide, for the first time, some insights into the anaphylactic miRNA serum profile in children and point to miR-21-3p and miR-487b-3p as candidate biomarkers. Furthermore, the SBA revealed a possible implication of these molecules in the underlying molecular mechanisms. Moreover, ECs increased miR-21-3p intracellularly and released it to the environment in response to anaphylaxis.
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