Evidence map›Paper›PMID 33875790›Full record

ArticleLaboratory investigation; a journal of technical methods and pathology2021

Extracellular vesicles derived from M2 microglia reduce ischemic brain injury through microRNA-135a-5p/TXNIP/NLRP3 axis.

Yue Liu, You-Ping Li, Li-Min Xiao, Li-Ke Chen, Su-Yue Zheng, Er-Ming Zeng, Chun-Hua Xu

Open access · bronzeAbstract read
PubMed Publisher
In one paragraph

Article in Laboratory investigation; a journal of technical methods and pathology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.

0numbers the graph read from it
0cells of the map it votes in
32citing papers in PubMed
2.8field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

32 citing papers in PubMed, 49 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Yue LiuDepartment of Neurosurgery, The First Affiliated Hospital of Nanchang University, Nanchang, PR China.
You-Ping LiDepartment of Neurosurgery, The First Affiliated Hospital of Nanchang University, Nanchang, PR China.
Li-Min XiaoDepartment of Neurosurgery, The First Affiliated Hospital of Nanchang University, Nanchang, PR China.
Li-Ke ChenDepartment of Neurosurgery, The First Affiliated Hospital of Nanchang University, Nanchang, PR China.
Su-Yue ZhengDepartment of Neurosurgery, The First Affiliated Hospital of Nanchang University, Nanchang, PR China.
Er-Ming ZengDepartment of Neurosurgery, The First Affiliated Hospital of Nanchang University, Nanchang, PR China.
Chun-Hua XuDepartment of Neurosurgery, The First Affiliated Hospital of Nanchang University, Nanchang, PR China. xu_chunhua67@163.com.ORCID 0000-0002-1468-4357
Nanchang University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Accumulating evidences have suggested that extracellular vesicles (EVs) are crucial players in the pathogenesis of ischemic brain injury. This study was designed to explore the specific functions of M2 phenotype microglia-derived EVs in ischemic brain injury progression. The expression of microRNA-135a-5p (miR-135a-5p) in M2 microglia-derived EVs was determined by reverse transcription-quantitative polymerase chain reaction (RT-qPCR), followed by the identification of expression relationship among miR-135a-5p, thioredoxin-interacting protein (TXNIP), and nod-like receptor protein 3 (NLRP3) by dual luciferase reporter gene assay. After construction of an oxygen-glucose deprivation/reperfusion (OGD/R) cell model, the effects of miR-135a-5p on the biological characteristics of HT-22 cells were assessed by cell counting kit 8 (CCK-8) assay and flow cytometry. Finally, a mouse model of transient middle cerebral artery occlusion (tMCAO) was established and cerebral infarction volume was determined by triphenyltetrazolium chloride (TTC) staining and the expression of IL-18 and IL-1β in the brain tissue was determined by enzyme-linked immunosorbent assay (ELISA). We found that M2 microglia-derived EVs had high expression of miR-135a-5p, and that miR-135a-5p in M2 microglia-derived EVs negatively regulated the expression of NLRP3 via TXNIP. Overexpression of miR-135a-5p promoted the proliferation but inhibited the apoptosis of neuronal cells, and inhibited the expression of autophagy-related proteins. M2 microglia-derived EVs delivered miR-135a-5p into neuronal cells to inhibit TXNIP expression, which further inhibited the activation of NLRP3 inflammasome, thereby reducing neuronal autophagy and ischemic brain injury. Hence, M2 microglia-derived EVs are novel therapeutic targets for ischemic brain injury treatment.

Indexed as

Extracellular VesiclesAnimalsBrain IschemiaCarrier ProteinsMaleMiceMice, Inbred ICRMicrogliaMicroRNAsNLR Family, Pyrin Domain-Containing 3 ProteinThioredoxinsCarrier ProteinsMicroRNAsMirn135 microRNA, mouseNLR Family, Pyrin Domain-Containing 3 ProteinNlrp3 protein, mouseThioredoxinsTxnip protein, mouse

Identifiers

PMID33875790
OpenAlexW3156700686

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.