ArticleJournal of the American Society of Nephrology : JASN2021
3D Mapping Reveals a Complex and Transient Interstitial Matrix During Murine Kidney Development.
Article in Journal of the American Society of Nephrology : JASN, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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25 citing papers in PubMed, 34 citations in OpenAlex.
- Decoding the Collagenome in Breast Cancer: Mechanotransduction, Microenvironment, and Translational Opportunities.International journal of molecular sciences · 2026Review
- Ageing impacts extracellular matrix turnover and remodelling in the kidney.Matrix biology plus · 2026Article
- Accelerated basement membrane remodeling and serum matrix fragments as biomarkers of fibrosis in Alport syndrome.Cell reports · 2026Article
- Update on Alport Syndrome: The Report of the 2024 International Workshop on Alport Syndrome.Kidney international reports · 2026Article
- The extracellular matrix: structure, composition, biological functions, diseases, and therapeutic targets.Molecular biomedicine · 2026Review
- Genetic diversity and differentiated adaptive strategies for underrepresented populations at the crossroad of Southeast and East Asia.BMC genomics · 2026Article
- Cryoelectrospun Elastin-Alginate Scaffolds Support In Vitro 3D Epithelial-Stromal Cocultures for Salivary Tissue Engineering.Gels (Basel, Switzerland) · 2025Article
- The kidney stroma in development and disease.Nature reviews. Nephrology · 2025Review
- Engineering kidney developmental trajectory using culture boundary conditions.Nature communications · 2025Article
- Extracellular matrix deposition precedes muscle-tendon integration during murine forelimb morphogenesis.Communications biology · 2025Article
- Cryoelectrospun elastin-alginate scaffolds as potential cell delivery vehicles for mesenchymal stromal cell therapy.Scientific reports · 2025Article
- Breaking the symmetry of cell contractility drives tubulogenesis via CXCL1 polarization.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Proteomic analysis of decellularized mice liver and kidney extracellular matrices.Journal of biological engineering · 2024Article
- Extracellular matrix protein composition dynamically changes during murine forelimb development.iScience · 2024Article
- Multi-omic analysis of human kidney tissue identified medulla-specific gene expression patterns.Kidney international · 2024Article
- Recent advances in extracellular matrix manipulation for kidney organoid research.Frontiers in pharmacology · 2024Review
- In Vivo Assessment of Laboratory-Grown Kidney Tissue Grafts.Bioengineering (Basel, Switzerland) · 2023Article
- Identification and Characterization of the Wilms Tumor Cancer Stem Cell.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Deciphering the Kidney Matrisome: Identification and Quantification of Renal Extracellular Matrix Proteins in Healthy Mice.International journal of molecular sciences · 2023Article
- The interstitium at the developing nephron in the fetal kidney during advanced pregnancy - a microanatomical inventory.Molecular and cellular pediatrics · 2022Article
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5 authors at 3 institutions in 1 country.
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Abstract
backgroundThe extracellular matrix (ECM) is a network of proteins and glycosaminoglycans that provides structural and biochemical cues to cells. In the kidney, the ECM is critical for nephrogenesis; however, the dynamics of ECM composition and how it relates to 3D structure during development is unknown.
methodsUsing embryonic day 14.5 (E14.5), E18.5, postnatal day 3 (P3), and adult kidneys, we fractionated proteins based on differential solubilities, performed liquid chromatography-tandem mass spectrometry, and identified changes in ECM protein content (matrisome). Decellularized kidneys were stained for ECM proteins and imaged in 3D using confocal microscopy.
resultsWe observed an increase in interstitial ECM that connects the stromal mesenchyme to the basement membrane (TNXB, COL6A1, COL6A2, COL6A3) between the embryo and adult, and a transient elevation of interstitial matrix proteins (COL5A2, COL12A1, COL26A1, ELN, EMID1, FBN1, LTBP4, THSD4) at perinatal time points. Basement membrane proteins critical for metanephric induction (FRAS1, FREM2) were highest in abundance in the embryo, whereas proteins necessary for integrity of the glomerular basement membrane (COL4A3, COL4A4, COL4A5, LAMB2) were more abundant in the adult. 3D visualization revealed a complex interstitial matrix that dramatically changed over development, including the perinatal formation of fibrillar structures that appear to support the medullary rays.
conclusionBy correlating 3D ECM spatiotemporal organization with global protein abundance, we revealed novel changes in the interstitial matrix during kidney development. This new information regarding the ECM in developing kidneys offers the potential to inform the design of regenerative scaffolds that can guide nephrogenesis
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