ArticleThe New phytologist2004
High rooting frequency and functional analysis of GUS and GFP expression in transgenic Medicago truncatula A17.
Article in The New phytologist, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
8 citing papers in PubMed, 39 citations in OpenAlex.
- Striga parasitizes transgenic hairy roots of Zea mays and provides a tool for studying plant-plant interactions.Plant methods · 2012Article
- The M. truncatula SUNN gene is expressed in vascular tissue, similarly to RDN1, consistent with the role of these nodulation regulation genes in long distance signaling.Plant signaling & behavior · 2012Article
- Backbone-free transformation of barrel medic (Medicago truncatula) with a Medicago-derived transfer DNA.Plant cell reports · 2010Article
- Production of transgenic barrel medic (Medicago truncatula Gaernt.) using the ipt-type MAT vector system and impairment of Recombinase-mediated excision events.Plant cell reports · 2009Article
- Composite Medicago truncatula plants harbouring Agrobacterium rhizogenes-transformed roots reveal normal mycorrhization by Glomus intraradices.Journal of experimental botany · 2009Article
- Recent Advances in Medicago truncatula Genomics.International journal of plant genomics · 2008Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
• An effective transformation method is described for Medicago truncatula A17, verifying its suitability as a model legume for functional genomics. • Media and culture methods are detailed that yielded an average frequency of 35% for recovery of transgenic shoots from cotyledonary node explants and 39% for root induction and regeneration of entire plants from 419 phosphinothricin-resistant shoots. • Fertile plants transgenic for both 35S-GFP and phas-GUS were obtained in five of eight independent experiments. The presence and stable inheritance of transgenes was confirmed by GFP or GUS expression and by genomic DNA blots. GFP expression driven by the normally constitutive CaMV 35S promoter diminished as the leaves matured. Although GUS was very strongly and uniformly expressed in seed cotyledons of most lines, one line exhibited an aberrant, patchy pattern. Additionally, weak GUS expression was evident in leaf veins from the normally stringently spatially regulated phas promoter. • Stably transformed, fertile, M. truncatula A17 plants were generated. The unconventional expression patterns for 35S-GFP and phas-GUS expression obtained in some transformants suggest the occurrence of novel epigenetic events.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.