ArticleThe New phytologist2004
Distinct regulation of sucrose: sucrose-1-fructosyltransferase (1-SST) and sucrose: fructan-6-fructosyltransferase (6-SFT), the key enzymes of fructan synthesis in barley leaves: 1-SST as the pacemaker.
Article in The New phytologist, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 66 citations in OpenAlex.
- Extensive sucrose cycling through the fructan pool of a C3 grass across a 200 to 800 μmol mol-1 atmospheric CO2 gradient.Plant physiology · 2026Article
- Validation of a QTL on Chromosome 1DS Showing a Major Effect on Salt Tolerance in Winter Wheat.International journal of molecular sciences · 2022Article
- High-Throughput Extraction and Enzymatic Determination of Sugars and Fructans in Fructan-Accumulating Plants.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Whole-Transcriptome Analysis Unveils the Synchronized Activities of Genes for Fructans in Developing Tubers of the Jerusalem Artichoke.Frontiers in plant science · 2020Article
- Physicochemical Composition and Apparent Degree of Polymerization of Fructans in Five Wild Agave Varieties: Potential Industrial Use.Foods (Basel, Switzerland) · 2019Article
- Article
- Article
- Spatiotemporal Dynamics of Oligofructan Metabolism and Suggested Functions in Developing Cereal Grains.Frontiers in plant science · 2015Review
- Cloning and characterization of a novel fructan 6-exohydrolase strongly inhibited by sucrose in Lolium perenne.Planta · 2014Article
- Article
- TaMYB13-1, a R2R3 MYB transcription factor, regulates the fructan synthetic pathway and contributes to enhanced fructan accumulation in bread wheat.Journal of experimental botany · 2013Article
- Dissecting the molecular basis of the contribution of source strength to high fructan accumulation in wheat.Plant molecular biology · 2013Article
- Clusters of genes encoding fructan biosynthesizing enzymes in wheat and barley.Plant molecular biology · 2012Article
- Significance of light, sugar, and amino acid supply for diurnal gene regulation in developing barley caryopses.Plant physiology · 2010Article
- Fructan and its relationship to abiotic stress tolerance in plants.Cellular and molecular life sciences : CMLS · 2009Review
- Large scale transcriptome analysis of the effects of nitrogen nutrition on accumulation of stem carbohydrate reserves in reproductive stage wheat.Plant molecular biology · 2008Article
- Fructan:fructan 1-fructosyltransferase, a key enzyme for biosynthesis of graminan oligomers in hardened wheat.Planta · 2005Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
• Previously we have cloned sucrose: fructan-6-fructosyltransferase (6-SFT) from barley (Hordeum vulgare) and proposed that synthesis of fructans in grasses depends on the concerted action of two main enzymes: sucrose: sucrose-1-fructosyltransferase (1-SST), as in other fructan producing plants, and 6-SFT, found only in grasses. • Here we report the cloning of barley 1-SST, verifying the activity of the encoded protein by expression in Pichia pastoris. As expected, the barley 1-SST is homologous to invertases and fructosyltransferases, and in particular to barley 6-SFT. • The gene expression pattern of 1-SST and 6-SFT, along with the corresponding enzyme activities and fructan levels, were investigated in excised barley leaves subjected to a light-dark regime known to sequentially induce fructan accumulation and mobilization. The turnover of transcripts and enzyme activities of 1-SST and 6-SFT was compared, using appropriate inhibitors. • We found the 1-SST transcripts and enzymatic activity respond quickly, being subject to a rapid turnover. By contrast, the 6-SFT transcripts and enzymatic activity were found to be much more stable. The much higher responsiveness of 1-SST to regulatory processes, as compared with 6-SFT, clearly indicates that 1-SST plays the role of the pacemaker enzyme of fructan synthesis in barley leaves.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.