ArticleThe New phytologist2004
Molecular genetic analysis of pollen irradiation mutagenesis in Arabidopsis.
Article in The New phytologist, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
5 citing papers in PubMed, 21 citations in OpenAlex.
- Induced and natural variation affect traits independently in hybrid Populus.G3 (Bethesda, Md.) · 2024Article
- Optimized methods for random and targeted mutagenesis in field pea (Frontiers in plant science · 2022Article
- Induced parthenogenesis by gamma-irradiated pollen in loquat for haploid production.Breeding science · 2016Article
- Modulating crossover positioning by introducing large structural changes in chromosomes.BMC genomics · 2015Article
- Induced parthenogenesis in mandarin for haploid production: induction procedures and genetic analysis of plantlets.Plant cell reports · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
• Pollen γ-irradiation mutagenesis was systematically investigated to generate targeted mutations in Arabidopsis. • Irradiation effects on viability, germination and frequency of loci deletions were evaluated. • Mutation frequency increased with irradiation dose and varied depending upon pollen developmental stage. Meiosis was the most irradiation-sensitive stage, however, it did not equate to the highest mutation frequency. High frequencies of targeted mutations were obtained by irradiation from the second mitosis to mature pollen stages, using 400-600 Gy. Targeted mutations could also be obtained using lower doses of γ-rays (e.g. 200 Gy) provided that pollen was irradiated at an earlier developmental stage. ms1ttg marker locus pseudo-dominants were used to verify the presence and size of the resultant deletions. • The results demonstrate that γ-irradiation of pollen is an efficient approach for generating deletions in the Arabidopsis genome. Pollen mutagenesis offers the possibility of combining single-cell selection procedures with the advantages of haploid systems, including the ability to treat large numbers of pollen grains, the absence of chimerism and direct expression of targeted alleles in the M
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