ReviewBioconjugate chemistry2021
Protein Delivery: If Your GFP (or Other Small Protein) Is in the Cytosol, It Will Also Be in the Nucleus.
Review in Bioconjugate chemistry, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 37 citations in OpenAlex.
- The divergent V2 protein encoded by parsley yellow leaf curl virus is a suppressor of transcriptional and post-transcriptional gene silencing and a potential symptom determinant.BMC plant biology · 2026Article
- Article
- Efficient Direct Cytosolic Protein Delivery via Protein-Linker Co-engineering.ACS applied materials & interfaces · 2025Article
- The fate of artificial transgenes in Acanthamoeba castellanii.BMC genomics · 2025Article
- Identification of a Nuclear Localization Signal (NLS) in Human Transcription Elongation Factor ELL2.Cell biochemistry and function · 2024Article
- Article
- Hitchhiking of Cas9 with nucleus-localized proteins impairs its controllability and leads to efficient genome editing of NLS-free Cas9.Molecular therapy : the journal of the American Society of Gene Therapy · 2024Article
- Filamentous-Actin-Mimicking Nanoplatform for Enhanced Cytosolic Protein Delivery.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Engineered Polymer-siRNA Polyplexes Provide Effective Treatment of Lung Inflammation.ACS nano · 2023Article
- A light-activated polymer with excellent serum tolerance for intracellular protein delivery.Chemical science · 2023Article
- Direct Cytosolic Delivery of Citraconylated Proteins.Pharmaceutics · 2023Article
- Protein charge parameters that influence stability and cellular internalization of polyelectrolyte complex micelles.Chemical science · 2022Article
- Structure-Uptake Relationship Study of DABCYL Derivatives Linked to Cyclic Cell-Penetrating Peptides for Live-Cell Delivery of Synthetic Proteins.Angewandte Chemie (International ed. in English) · 2022Article
- Tyrosine bioconjugation with hypervalent iodine.Chemical science · 2022Article
- Far-Red Fluorescent Murine Glioma Model for Accurate Assessment of Brain Tumor Progression.Cancers · 2022Article
- Probing the cell delivery of synthetic diubiquitin chains.Chemical communications (Cambridge, England) · 2022Article
- Determining the Cytosolic Stability of Small DNA NanostructuresNano letters · 2022Article
- Direct Cytosolic Delivery of Proteins Using Lyophilized and Reconstituted Polymer-Protein Assemblies.Pharmaceutical research · 2022Article
- Cytosolic Protein Delivery Using Modular Biotin-Streptavidin Assembly of Nanocomposites.ACS nano · 2022Article
- Genetic and Covalent Protein Modification Strategies to Facilitate Intracellular Delivery.Biomacromolecules · 2021Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 2 countries.
Funding
Abstract
Intracellular protein delivery is a transformative tool for biologics research and medicine. Delivery into the cytosol allows proteins to diffuse throughout the cell and access subcellular organelles. Inefficient delivery caused by endosomal entrapment is often misidentified as cytosolic delivery. This inaccuracy muddles what should be a key checkpoint in assessing delivery efficiency. Green fluorescent protein (GFP) is a robust cargo small enough to passively diffuse from the cytosol into the nucleus. Fluorescence of GFP in the nucleus is a direct readout for cytosolic access and effective delivery. Here, we highlight recent examples from the literature for the accurate assessment of cytosolic protein delivery using GFP fluorescence in the cytosol and nucleus.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.