ArticleNucleic acids research2021
Intermolecular dark resonance energy transfer (DRET): upgrading fluorogenic DNA sensing.
Article in Nucleic acids research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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9 citing papers in PubMed, 15 citations in OpenAlex.
- Microenvironment-responsive tetrahedral DNA nanoplatform for specific and enhanced tumor therapy through differential ROS and angiogenesis modulation.Journal of nanobiotechnology · 2026Article
- A Metallosupramolecular Receptor for Squaraine Dyes Enabling Ultrafast Dark Resonance Energy Transfer.Angewandte Chemie (International ed. in English) · 2026Article
- Intermolecular dark resonance energy transfer (DRET) for high contrast imaging of endogenous mRNAs in 3D biological samples.Scientific reports · 2025Article
- Acidity-activatable upconversion afterglow luminescence cocktail nanoparticles for ultrasensitive in vivo imaging.Nature communications · 2024Article
- The potential of fluorogenicity for single molecule FRET and DyeCycling.QRB discovery · 2024Review
- A Robust and Efficient FRET-Based Assay for Cannabinoid Receptor Ligands Discovery.Molecules (Basel, Switzerland) · 2023Article
- Ruthenium(II) Polypyridyl Complexes as FRET Donors: Structure- and Sequence-Selective DNA-Binding and Anticancer Properties.Journal of the American Chemical Society · 2023Article
- Hybridization-Sensitive Fluorescent Probes for DNA and RNA by a Modular "Click" Approach.Bioconjugate chemistry · 2022Article
- Functionalization of Biphenylcarbazole (CBP) with Siloxane-Hybrid Chains for Solvent-Free Liquid Materials.Molecules (Basel, Switzerland) · 2021Article
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The sensitivity of FRET-based sensing is usually limited by the spectral overlaps of the FRET donor and acceptor, which generate a poor signal-to-noise ratio. To overcome this limitation, a quenched donor presenting a large Stokes shift can be combined with a bright acceptor to perform Dark Resonance Energy Transfer (DRET). The consequent fluorogenic response from the acceptor considerably improves the signal-to-noise ratio. To date, DRET has mainly relied on a donor that is covalently bound to the acceptor. In this context, our aim was to develop the first intermolecular DRET pair for specific sensing of nucleic acid sequences. To this end, we designed DFK, a push-pull probe based on a fluorenyl π-platform that is strongly quenched in water. DFK was incorporated into a series of oligonucleotides and used as a DRET donor with Cy5-labeled complementary sequences. In line with our expectations, excitation of the dark donor in the double-labeled duplex switched on the far-red Cy5 emission and remained free of cross-excitation. The DRET mechanism was supported by time-resolved fluorescence measurements. This concept was then applied with binary probes, which confirmed the distance dependence of DRET as well as its potency in detecting sequences of interest with low background noise.
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