Evidence map›Paper›PMID 33872071›Full record

ArticleThe American journal of sports medicine2021

Ascorbic Acid and Iron Supplement Treatment Improves Stem Cell-Mediated Cartilage Regeneration in a Minipig Model.

Ashok Joseph Theruvath, Elhussein Elbadry Mahmoud, Wei Wu, Hossein Nejadnik, Louise Kiru, Tie Liang, Stephen Felt, Heike Elisabeth Daldrup-Link

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The American journal of sports medicine, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
3.1field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 27 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 2 countries.

Ashok Joseph TheruvathMolecular Imaging Program at Stanford, Department of Radiology, School of Medicine, Stanford University, Stanford, California, USA.
Elhussein Elbadry MahmoudMolecular Imaging Program at Stanford, Department of Radiology, School of Medicine, Stanford University, Stanford, California, USA.
Wei WuMolecular Imaging Program at Stanford, Department of Radiology, School of Medicine, Stanford University, Stanford, California, USA.
Hossein NejadnikMolecular Imaging Program at Stanford, Department of Radiology, School of Medicine, Stanford University, Stanford, California, USA.
Louise KiruMolecular Imaging Program at Stanford, Department of Radiology, School of Medicine, Stanford University, Stanford, California, USA.
Tie LiangMolecular Imaging Program at Stanford, Department of Radiology, School of Medicine, Stanford University, Stanford, California, USA.
Stephen FeltDepartment of Comparative Medicine, School of Medicine, Stanford University, Stanford, California, USA.
Heike Elisabeth Daldrup-LinkMolecular Imaging Program at Stanford, Department of Radiology, School of Medicine, Stanford University, Stanford, California, USA.
Stanford University · USSouth Valley University · EG

Funding

Monitoring of Stem Cell Engraftment in Arthritic Joints with MR ImagingR01AR054458 · NIAMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI DALDRUP-LINK, HEIKE ELIZABETH · 2008 to 2021
$6.7M
Personalized Whole Body Staging for Children with Cancer: A Solution to the Conundrum of Long-Term Side Effects from CT and PET/CT ScansR01HD081123 · NICHD · STANFORD UNIVERSITY · PI DALDRUP-LINK, HEIKE ELIZABETH · 2015 to 2019
$2.5M
NIAMS NIH HHS R01 AR054458NICHD NIH HHS R01 HD081123
6 · The paper itself

Abstract

backgroundThe transplantation of mesenchymal stem cells (MSCs) into cartilage defects has led to variable cartilage repair outcomes. Previous in vitro studies have shown that ascorbic acid and reduced iron independently can improve the chondrogenic differentiation of MSCs. However, the combined effect of ascorbic acid and iron supplementation on MSC differentiation has not been investigated. PURPOSE: To investigate the combined in vivo effects of ascorbic acid and a US Food and Drug Administration (FDA)-approved iron supplement on MSC-mediated cartilage repair in mature Göttingen minipigs. STUDY

designControlled laboratory study.

methodsWe pretreated bone marrow-derived MSCs with ascorbic acid and the FDA-approved iron supplement ferumoxytol and then transplanted the MSCs into full-thickness cartilage defects in the distal femurs of Göttingen minipigs. Untreated cartilage defects served as negative controls. We evaluated the cartilage repair site with magnetic resonance imaging at 4 and 12 weeks after MSC implantation, followed by histological examination and immunofluorescence staining at 12 weeks.

resultsAscorbic acid plus iron-pretreated MSCs demonstrated a significantly better MOCART (magnetic resonance observation of cartilage repair tissue) score (73.8 ± 15.5), better macroscopic cartilage regeneration score according to the International Cartilage Repair Society (8.6 ± 2.0), better Pineda score (2.9 ± 0.8), and larger amount of collagen type II (28,469 ± 21,313) compared with untreated controls (41.3 ± 2.5, 1.8 ± 2.9, 12.8 ± 1.9, and 905 ± 1326, respectively). The obtained scores were also better than scores previously reported in the same animal model for MSC implants without ascorbic acid.

conclusionPretreatment of MSCs with ascorbic acid and an FDA-approved iron supplement improved the chondrogenesis of MSCs and led to hyaline-like cartilage regeneration in the knee joints of minipigs. CLINICAL RELEVANCE: Ascorbic acid and iron supplements are immediately clinically applicable. Thus, these results, in principle, could be translated into clinical applications.

Indexed as

Cartilage, ArticularMesenchymal Stem CellsMesenchymal Stem Cell TransplantationAnimalsAscorbic AcidCell DifferentiationChondrogenesisIronSwineSwine, MiniatureAscorbic AcidIronascorbic acidcartilage repairchondrogenesisminipigMRIstem cells

Identifiers

PMID33872071
PMCPMC8177720
OpenAlexW3154050831

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.