ArticleJournal of visualized experiments : JoVE2021
Generation and Culture of Lingual Organoids Derived from Adult Mouse Taste Stem Cells.
Article in Journal of visualized experiments : JoVE, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 15 citations in OpenAlex.
- Tyrosine kinase inhibitors affect sweet taste and dysregulate fate selection of specific taste bud cell subtypes via KIT inhibition.Development (Cambridge, England) · 2026Article
- Tripotent Lgr5 stem cells in the posterior tongue generate lingual, taste, and salivary gland lineages.Nature communications · 2025Article
- Pig Taste Cell-derived Organoids Synthesize Insulin.Endocrinology · 2025Article
- Organoid models for chemosensing cell studies.American journal of physiology. Cell physiology · 2025Review
- BuZhong YiQi Formula Alleviates Taste Disorders in Rats with Type 2 Diabetes Mellitus by Increasing the Number of Taste Buds and the Expression of Signaling Molecules in Taste Transduction Pathways.Pharmaceuticals (Basel, Switzerland) · 2025Article
- Advances in cell-based biosensors: Transforming food flavor evaluation with novel approaches.Food chemistry: X · 2025Review
- Developing and regenerating a sense of taste.Current topics in developmental biology · 2025Review
- Longitudinal imaging of the taste bud in vivo with two-photon laser scanning microscopy.PloS one · 2024Article
- Physiology of the tongue with emphasis on taste transduction.Physiological reviews · 2023Review
- High Sox2 expression predicts taste lineage competency of lingual progenitors in vitro.Development (Cambridge, England) · 2023Article
- The sense of taste: Development, regeneration, and dysfunction.WIREs mechanisms of disease · 2022Review
- 3D and organoid culture in research: physiology, hereditary genetic diseases and cancer.Cell & bioscience · 2022Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
The sense of taste is mediated by taste buds on the tongue, which are composed of rapidly renewing taste receptor cells (TRCs). This continual turnover is powered by local progenitor cells and renders taste function prone to disruption by a multitude of medical treatments, which in turn severely impacts the quality of life. Thus, studying this process in the context of drug treatment is vital to understanding if and how taste progenitor function and TRC production are affected. Given the ethical concerns and limited availability of human taste tissue, mouse models, which have a taste system similar to humans, are commonly used. Compared to in vivo methods, which are time-consuming, expensive, and not amenable to high throughput studies, murine lingual organoids can enable experiments to be run rapidly with many replicates and fewer mice. Here, previously published protocols have been adapted and a standardized method for generating taste organoids from taste progenitor cells isolated from the circumvallate papilla (CVP) of adult mice is presented. Taste progenitor cells in the CVP express LGR5 and can be isolated via EGFP fluorescence-activated cell sorting (FACS) from mice carrying an Lgr5
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.