Evidence map›Paper›PMID 33870224›Full record

ArticleSTAR protocols2021

Identifying cytokine signaling signatures in primary human Th-1 cells by phospho-proteomics analysis.

Jonathan Martinez-Fabregas, Elizabeth Pohler, Ignacio Moraga

Open access · goldAbstract read
In one paragraph

Article in STAR protocols, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 97% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Jonathan Martinez-FabregasDivision of Cell Signaling and Immunology, School of Life Sciences, University of Dundee, Dundee, UK.
Elizabeth PohlerDivision of Cell Signaling and Immunology, School of Life Sciences, University of Dundee, Dundee, UK.
Ignacio MoragaDivision of Cell Signaling and Immunology, School of Life Sciences, University of Dundee, Dundee, UK.
University of Dundee · GB

Funding

Wellcome Trust 02323/Z/16/Z
6 · The paper itself

Abstract

Stable isotope labeling by amino acid-based high-resolution phosphoproteomics is a powerful technique that allows for direct comparison of cells stimulated under different experimental conditions. This feature makes it the ideal methodology to identify cytokine signaling networks. Here, we present an optimized protocol for the isolation and identification of phosphopeptides from IL-6-stimulated primary human Th-1 cells. For complete details on the use and execution of this protocol, please refer to Martinez-Fabregas et al. (2020).

Indexed as

PhosphoproteinsCells, CulturedCytokinesHumansProteomeProteomicsSignal TransductionTh1 CellsCytokinesPhosphoproteinsProteomeCell isolationImmunologyProtein BiochemistryProteomicsSignal Transduction

Identifiers

PMID33870224
PMCPMC8044715
OpenAlexW3146535823

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.