Evidence map›Paper›PMID 33870157›Full record

ArticleJHEP reports : innovation in hepatology2021

Whole genome deep sequencing analysis of viral quasispecies diversity and evolution in HBeAg seroconverters.

Su-Ru Lin, Ta-Yu Yang, Cheng-Yuan Peng, You-Yu Lin, Chia-Yen Dai, Hurng-Yi Wang, Tung-Hung Su, Tai-Chung Tseng, I-Jung Liu, Huei-Ru Cheng and 7 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in JHEP reports : innovation in hepatology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
2.0field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 13 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors at 5 institutions in 1 country.

Su-Ru LinDepartment of Microbiology, National Taiwan University, Taipei, Taiwan.
Ta-Yu YangDepartment of Microbiology, National Taiwan University, Taipei, Taiwan.
Cheng-Yuan PengSchool of Medicine, China Medical University, Taichung, Taiwan.
You-Yu LinGraduate Institute of Clinical Medicine, College of Medicine, National Taiwan University, Taipei, Taiwan.
Chia-Yen DaiDepartment of Internal Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.
Hurng-Yi WangGraduate Institute of Clinical Medicine, College of Medicine, National Taiwan University, Taipei, Taiwan.
Tung-Hung SuDivision of Gastroenterology and Hepatology, Department of Internal Medicine, National Taiwan University Hospital, Taipei, Taiwan.
Tai-Chung TsengDivision of Gastroenterology and Hepatology, Department of Internal Medicine, National Taiwan University Hospital, Taipei, Taiwan.
I-Jung LiuCardinal Tien Junior College of Healthcare and Management, New Taipei City, Taiwan.
Huei-Ru ChengGraduate Institute of Clinical Medicine, College of Medicine, National Taiwan University, Taipei, Taiwan.
Yueh-Chi ShenDepartment of Microbiology, National Taiwan University, Taipei, Taiwan.
Fang-Yi WuDepartment of Microbiology, National Taiwan University, Taipei, Taiwan.
Chun-Jen LiuGraduate Institute of Clinical Medicine, College of Medicine, National Taiwan University, Taipei, Taiwan.
Ding-Shinn ChenGraduate Institute of Clinical Medicine, College of Medicine, National Taiwan University, Taipei, Taiwan.
Pei-Jer ChenGraduate Institute of Clinical Medicine, College of Medicine, National Taiwan University, Taipei, Taiwan.
Hung-Chih YangDepartment of Microbiology, National Taiwan University, Taipei, Taiwan.
Jia-Horng KaoGraduate Institute of Clinical Medicine, College of Medicine, National Taiwan University, Taipei, Taiwan.
National Taiwan University · TWNational Taiwan University Hospital · TWCardinal Tien College of Healthcare and Management · TWChina Medical University Hospital · TWKaohsiung Medical University · TW

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

BACKGROUND &

aimsWe aimed to investigate how viral quasispecies of the HBV whole genome evolves and diversifies in response to HBeAg seroconversion and viral control utilising next-generation sequencing (NGS).

methodsFifty HBeAg-positive chronic hepatitis B patients, including 18 treatment-naïve and 32 interferon (IFN)-treated individuals, were recruited. Serial HBV whole genomes in serum were analysed by NGS to determine sequence characteristics and viral quasispecies.

resultsHBV quasispecies diversity, measured by nucleotide diversity, was negatively correlated with viral load and hepatitis activity. Spontaneous HBeAg seroconverters exhibited significantly greater viral quasispecies diversity than treatment-naïve non-seroconverters from >1 year before seroconversion (0.0112

conclusionsHigher HBV quasispecies diversity is associated with spontaneous HBeAg seroconversion and IFN-induced HBeAg seroconversion with low viraemia, conferring a favourable clinical outcome. LAY SUMMARY: HBeAg seroconversion is a landmark in the natural history of chronic HBV infection. Using next-generation sequencing, we found that the nucleotide diversity of HBV was negatively correlated with viral load and hepatitis activity. Patients undergoing HBeAg seroconversion had more diverse HBV genomes and a faster viral evolution rate. Our findings suggest HBeAg seroconversion is driven by host selection pressure, likely immune selection pressure.

Indexed as

ALT, alanine aminotransferaseAUC, area under curveBCP, basal core promoterC, coreCHB, chronic hepatitis BChronic hepatitis BdN, nonsynonymous substitution ratedS, synonymous substitution rateEOT, end of treatmentHBeAg seroconversionIFN-eSC, IFN-treated HBeAg seroconvertersIFN, interferonIFN-No-eSC, IFN-treated HBeAg non-seroconvertersIFN-NR, IFN-non-respondersIFN-RS, IFN-respondersIntra-host single nucleotide variantsiSNVs, intra-host single-nucleotide variantsNGS, next-generation sequencingORFs, open reading framesP, polymeraseS, surfaceTN-eSC, treatment-naïve HBeAg seroconvertersTN-No-eSC, treatment-naïve non-seroconverters

Identifiers

PMID33870157
PMCPMC8042178
OpenAlexW3131792063

What OpenQuestion holds

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LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.