ArticleiScience2021
NASA GeneLab RNA-seq consensus pipeline: standardized processing of short-read RNA-seq data.
Article in iScience, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
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Who cites it
35 citing papers in PubMed.
- Hyperspectral imaging reveals early drought stress and associated molecular responses in lettuce for space agriculture.Plant phenomics (Washington, D.C.) · 2026Article
- Aging in Spaceflight, Oxidative Stress, and Microbiome Dysbiosis: A Comprehensive Review.International journal of molecular sciences · 2026Review
- ibSLS as a biobank for democratizing access to multi-omics data and biospecimens from spaceflight research.Nature communications · 2026Article
- Automated behavioral segmentation and markerless pose tracking of mice during spaceflight.bioRxiv : the preprint server for biology · 2026Article
- Article
- Stress and light spectral quality influence the transcriptome of a tomato crop on the International Space Station.BMC plant biology · 2026Article
- Multiscale Modeling and Systems Biology in Microgravity Investigations.Methods in molecular biology (Clifton, N.J.) · 2026Article
- SLC25A mitochondrial carriers as biomarkers and therapeutic targets of spaceflight-induced dysfunction: the ADP/ATP carrier (AAC3) as a structural case study.Journal of translational medicine · 2025Article
- Commentary: a review of technical considerations for planning an RNA-Sequencing experiment.BMC genomics · 2025Review
- Oviductal extracellular matrix hydrogels enhance in vitro culture of rabbit embryos and reduce deficiencies during assisted reproductive technologies.Scientific reports · 2024Article
- Epstein-Barr virus induces host shutoff extensively via BGLF5-independent mechanisms.Cell reports · 2024Article
- Local cryptic diversity in salinity adaptation mechanisms in the wild outcrossingProceedings of the National Academy of Sciences of the United States of America · 2024Article
- 3t-seq: automatic gene expression analysis of single-copy genes, transposable elements, and tRNAs from RNA-seq data.Briefings in bioinformatics · 2024Article
- Omics Studies of Specialized Cells and Stem Cells under Microgravity Conditions.International journal of molecular sciences · 2024Review
- Spaceflight alters host-gut microbiota interactions.NPJ biofilms and microbiomes · 2024Article
- Transcriptomic Signatures of the Foetal Liver and Late Prenatal Development in Vitrified Rabbit Embryos.Veterinary sciences · 2024Article
- Harmonizing heterogeneous transcriptomics datasets for machine learning-based analysis to identify spaceflown murine liver-specific changes.NPJ microgravity · 2024Article
- Direct RNA sequencing of astronaut blood reveals spaceflight-associated m6A increases and hematopoietic transcriptional responses.Nature communications · 2024Article
- Transcriptomics analysis reveals molecular alterations underpinning spaceflight dermatology.Communications medicine · 2024Article
- Explainable machine learning identifies multi-omics signatures of muscle response to spaceflight in mice.NPJ microgravity · 2023Article
Corrections and comments
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Authors and funding
45 authors.
Funding
Abstract
With the development of transcriptomic technologies, we are able to quantify precise changes in gene expression profiles from astronauts and other organisms exposed to spaceflight. Members of NASA GeneLab and GeneLab-associated analysis working groups (AWGs) have developed a consensus pipeline for analyzing short-read RNA-sequencing data from spaceflight-associated experiments. The pipeline includes quality control, read trimming, mapping, and gene quantification steps, culminating in the detection of differentially expressed genes. This data analysis pipeline and the results of its execution using data submitted to GeneLab are now all publicly available through the GeneLab database. We present here the full details and rationale for the construction of this pipeline in order to promote transparency, reproducibility, and reusability of pipeline data; to provide a template for data processing of future spaceflight-relevant datasets; and to encourage cross-analysis of data from other databases with the data available in GeneLab.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.