ArticleiScience2021
Cryopreservation of microglia enables single-cell RNA sequencing with minimal effects on disease-related gene expression patterns.
Article in iScience, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 27 citations in OpenAlex.
- Selective under-representation of transcripts encoding membrane-integrated architectures following neural tissue vitrification.Biochemistry and biophysics reports · 2026Article
- Immune response to adeno-associated viral vectors in a human iPSC-derived microglia chimeric mouse model.Cell reports methods · 2026Article
- Methodologies for Sample Multiplexing and Computational Deconvolution in Single-Cell Sequencing.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- A validated method for banking mixed glial cultures that yield responsive microglia.bioRxiv : the preprint server for biology · 2025Article
- T cell-mediated SIV dissemination into the CNS: a single-cell transcriptomic analysis.Journal of neuroinflammation · 2025Article
- Opioid-induced immunosuppression of brain myeloid cells in SIV-infected rhesus macaques.Research square · 2025Article
- SIV infection induces alterations in gene expression and loss of interneurons in Rhesus Macaque frontal cortex during early systemic infection.Translational psychiatry · 2025Article
- Transformation of brain myeloid cell populations by SIV in rhesus macaques revealed by multiomics.Communications biology · 2025Article
- Transformation of brain myeloid cell populations by SIV in rhesus macaques revealed by multiomics.Research square · 2024Article
- Microglia and macrophages alterations in the CNS during acute SIV infection: A single-cell analysis in rhesus macaques.PLoS pathogens · 2024Article
- CHIT1 at diagnosis predicts faster disability progression and reflects early microglial activation in multiple sclerosis.Nature communications · 2024Article
- Microglia and macrophages alterations in the CNS during acute SIV infection: a single-cell analysis in rhesus macaques.bioRxiv : the preprint server for biology · 2024Article
- Cryopreservation of cerebrospinal fluid cells preserves the transcriptional landscape for single-cell analysis.Journal of neuroinflammation · 2024Article
- Antiretroviral Therapy Ameliorates Simian Immunodeficiency Virus-Associated Myocardial Inflammation by Dampening Interferon Signaling and Pathogen Response in the Heart.The Journal of infectious diseases · 2023Article
- Antiretroviral therapy restores the homeostatic state of microglia in SIV-infected rhesus macaques.Journal of leukocyte biology · 2022Article
- Comparison of single‑nucleus and single‑cell transcriptomes in hepatocellular carcinoma tissue.Molecular medicine reports · 2022Article
- Practical Considerations for Single-Cell Genomics.Current protocols · 2022Review
- Morphine suppresses peripheral responses and transforms brain myeloid gene expression to favor neuropathogenesis in SIV infection.Frontiers in immunology · 2022Article
- DropletQC: improved identification of empty droplets and damaged cells in single-cell RNA-seq data.Genome biology · 2021Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Microglia play a key role in brain development, normal homeostasis, and neurodegenerative disorders. Single-cell technologies have led to important findings about microglia, with many animal model studies using single-cell RNA sequencing (scRNA-seq), whereas most human specimen studies using archived frozen brains for single-nucleus RNA sequencing (snRNA-seq). However, microglia compose a small proportion of the total brain tissue; snRNAseq depletes expression of microglia activation genes that characterize many diseases. Here we examine the use of purified, cryopreserved microglia for scRNA-seq. Comparison of scRNA-seq on paired fresh and cryopreserved microglia from rhesus monkeys revealed a high level of correlation of gene expression between the two conditions. Disease-related genes were relatively unaffected, but an increase in immediate-early gene expression was present in cryopreserved cells. Regardless, changes in immediate-early gene expression are still detectable. Cryopreservation of microglia is a suitable procedure for prospectively archiving samples.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.