ArticleResearch and practice in thrombosis and haemostasis2021
Fluorescence artifact correction in the thrombin generation assay: Necessity for correction algorithms in procoagulant samples.
Article in Research and practice in thrombosis and haemostasis, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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Who cites it
2 citing papers in PubMed, 23 citations in OpenAlex.
- Single-Particle Plasmon Sensor to Monitor Proteolytic Activity in Real Time.ACS applied optical materials · 2023Article
- Sources of bias and limitations of thrombinography: inner filter effect and substrate depletion at the edge of failure algorithm.Thrombosis journal · 2023Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
introductionThe thrombin generation (TG) test is a global hemostasis assay sensitive to procoagulant conditions. However, some TG assays may underestimate elevated TG when the thrombin fluorogenic substrate is depleted or fluorescence is attenuated by the inner filter effect (IFE).
objectivesWe sought to elucidate the extent to which procoagulant conditions require correcting for fluorogenic substrate depletion and/or IFE.
methodsWe analyzed corrections for substrate depletion and IFE and their effect on TG parameters in plasma samples with elevated blood coagulation factors in the presence or absence of thrombomodulin via commercial calibrated automated thrombogram (CAT) platform and in-house software capable of internal thrombin calibration with or without CAT-like artifact correction.
resultsElevated thrombin peak height (TPH) and endogenous thrombin potential (ETP) were detected with 2× and 4× increases in blood coagulation factors I, V, VIII, IX, X, and XI, or prothrombin in the presence or absence of artifact correction. The effect of the CAT algorithm was evident in TG curves from both low procoagulant (thrombomodulin-supplemented) and procoagulant (factor-supplemented) plasma samples. However, in all samples, with the exception of elevated prothrombin, CAT's correction was small (<10%) and did not affect detection of procoagulant samples versus normal plasma. For elevated prothrombin samples, uncorrected TPH or ETP values were underestimated, and CAT correction produced drastically elevated TG curves.
conclusionsOur data suggest that correction for substrate consumption and IFE, as offered by the CAT algorithm, is critical for detecting a subset of extremely procoagulant samples, such as elevated prothrombin, but is not necessary for all other conditions, including elevated factors XI and VIII.
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