ArticlePlanta2021
Importation of taxadiene synthase into chloroplast improves taxadiene production in tobacco.
Article in Planta, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 20 citations in OpenAlex.
- Toward Sustainable Paclitaxel Bioproduction: Plant Biology, Biosynthesis and Platform Engineering.Plants (Basel, Switzerland) · 2026Review
- Editing and synthetic engineering of chloroplast genomes.Plant communications · 2026Review
- An efficient system for plastid transformation in an edible medicinal herb.Transgenic research · 2025Article
- Metabolic Engineering of Terpenoid Biosynthesis in Medicinal Plants: From Genomic Insights to Biotechnological Applications.Current issues in molecular biology · 2025Review
- A faster killing effect of plastid-mediated RNA interference on a leaf beetle through induced dysbiosis of the gut bacteria.Plant communications · 2024Article
- Control of two insect pests by expression of a mismatch corrected double-stranded RNA in plants.Plant biotechnology journal · 2024Article
- Heterologous expression of taxane genes confers resistance to fall armyworm in Nicotiana benthamiana.Plant cell reports · 2024Article
- Functions of Representative Terpenoids and Their Biosynthesis Mechanisms in Medicinal Plants.Biomolecules · 2023Review
- Research Advances in Clinical Applications, Anticancer Mechanism, Total Chemical Synthesis, Semi-Synthesis and Biosynthesis of Paclitaxel.Molecules (Basel, Switzerland) · 2023Review
- Genetic approaches in improving biotechnological production of taxanes: An update.Frontiers in plant science · 2023Review
- Review
- Efficient control of western flower thrips by plastid-mediated RNA interference.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
MAIN
conclusionImportation of taxadiene synthase into chloroplasts is important for the efficient heterologous production of taxadiene. Taxadiene, the first committed precursor to taxol, is synthesized from geranylgeranyl pyrophosphate (GGPP) by action of taxadiene synthase (TS). Heterologous production of taxadiene could potentially rely on both cytosolic mevalonic acid (MVA) pathway and the plastidic methylerythritol phosphate (MEP) pathway. We suggest the compartmentalized engineering in chloroplast as an efficient approach for taxadiene production. In this study, we directly introduced the TS gene from Taxus brevifolia into the tobacco chloroplast genome and found that the transplastomic plants accumulated a low content of taxadiene, ~ 5.6 μg/g dry weight (DW). Moreover, we tried a combination of MEP and MVA pathways for taxadiene synthesis by nuclear transformation with a truncated version of TS (without encoding a transit peptide) into the transplastomic plants. However, this did not further improve the taxadiene production. In contrast, we found that taxadiene could be produced up to 87.8 μg/g DW in leaves of transgenic plants expressing TS with a chloroplast transit peptide, which was significantly higher than that in leaves of transplastomic plants. Thus, this study highlights the importance of TS importation into chloroplast for production of taxadiene.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.